Brazilian artisanal cheeses as a source of beneficial Enterococcus faecium strains: characterization of the bacteriocinogenic potential
| العنوان: | Brazilian artisanal cheeses as a source of beneficial Enterococcus faecium strains: characterization of the bacteriocinogenic potential |
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| المؤلفون: | Jessica Catarine Frutuoso do Nascimento, Svetoslav Dimitrov Todorov, Juliane Döering Gasparin Carvalho, Cintia Renata Costa Rocha, Laura Maria Bruno, Ana Catarina de Souza Lopes, Karina Maria Olbrich dos Santos, Antônio Diogo Silva Vieira, Bernadette Dora Gombossy de Melo Franco |
| المصدر: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| بيانات النشر: | Springer Science and Business Media LLC, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | biology, Proteolytic enzymes, 16S ribosomal RNA, biology.organism_classification, medicine.disease_cause, Applied Microbiology and Biotechnology, Microbiology, Bacteriocin, Listeria monocytogenes, Catalase, biology.protein, medicine, MICROBIOLOGIA DE ALIMENTOS, Lipase, Enterococcus faecium |
| الوصف: | Two bacterial isolates from milk and cheeses were selected based on their inhibition of Listeria monocytogenes, and classified as Enterococcus faecium based on 16S rRNA analysis. In MRS broth at 37 °C, bacteriocin-like substances (BLS) produced by E. faecium EM485 and EM925 were detected at 3,200 arbitrary units/mL. These peptides were inactivated by proteolytic enzymes, but not when treated with α-amylase, catalase and lipase. The two BLS remained stable at pH values ranging from 2.0 to 10.0, after exposure to 100 °C for 120 min and in the presence of surfactants and salts. DNA from both strains generated positive PCR results for enterocin A and B genes. |
| تدمد: | 1869-2044 1590-4261 |
| DOI: | 10.1007/s13213-013-0789-4 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1bd7b016ed81cedea15f95f2a7e6f73a https://doi.org/10.1007/s13213-013-0789-4 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....1bd7b016ed81cedea15f95f2a7e6f73a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18692044 15904261 |
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| DOI: | 10.1007/s13213-013-0789-4 |