A cut above
| العنوان: | A cut above |
|---|---|
| المؤلفون: | Roberto Bonasio, Chongsheng He |
| المصدر: | eLife, Vol 6 (2017) eLife |
| بيانات النشر: | eLife Sciences Publications Ltd, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, QH301-705.5, Science, S. cerevisiae, Saccharomyces cerevisiae, Biology, General Biochemistry, Genetics and Molecular Biology, 03 medical and health sciences, transcription factors, in situ profiling, Humans, Micrococcal Nuclease, DNA sequencing, Biology (General), Molecular Biology, Genetics, Binding Sites, General Immunology and Microbiology, General Neuroscience, DNA, General Medicine, 030104 developmental biology, ComputingMethodologies_PATTERNRECOGNITION, Genes and Chromosomes, chromatin, Medicine, Insight, Algorithm, chromatin mapping, Human, Protein Binding |
| الوصف: | We describe Cleavage Under Targets and Release Using Nuclease (CUTRUN), a chromatin profiling strategy in which antibody-targeted controlled cleavage by micrococcal nuclease releases specific protein-DNA complexes into the supernatant for paired-end DNA sequencing. Unlike Chromatin Immunoprecipitation (ChIP), which fragments and solubilizes total chromatin, CUTRUN is performed in situ, allowing for both quantitative high-resolution chromatin mapping and probing of the local chromatin environment. When applied to yeast and human nuclei, CUTRUN yielded precise transcription factor profiles while avoiding crosslinking and solubilization issues. CUTRUN is simple to perform and is inherently robust, with extremely low backgrounds requiring only ~1/10th the sequencing depth as ChIP, making CUTRUN especially cost-effective for transcription factor and chromatin profiling. When used in conjunction with native ChIP-seq and applied to human CTCF, CUTRUN mapped directional long range contact sites at high resolution. We conclude that in situ mapping of protein-DNA interactions by CUTRUN is an attractive alternative to ChIP-seq. |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1b51bab846790e77274bf7bf1febabd9 https://elifesciences.org/articles/25000 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....1b51bab846790e77274bf7bf1febabd9 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |