Release of calcium from intracellular stores in rat basophilic leukemia cells monitored with the fluorescent probe chlortetracycline
| العنوان: | Release of calcium from intracellular stores in rat basophilic leukemia cells monitored with the fluorescent probe chlortetracycline |
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| المؤلفون: | Clare Fewtrell, Gregory V. Marcotte, Paul J. Millard |
| المصدر: | Journal of Cellular Physiology. 142:78-88 |
| بيانات النشر: | Wiley, 1990. |
| سنة النشر: | 1990 |
| مصطلحات موضوعية: | Chlortetracycline, Physiology, Clinical Biochemistry, Antimycin A, chemistry.chemical_element, Oxytetracycline, Calcium, Biology, Calcium in biology, Exocytosis, chemistry.chemical_compound, medicine, Animals, Magnesium, Egtazic Acid, Fluorescent Dyes, Calcium metabolism, Ionomycin, Cell Biology, Rats, EGTA, Spectrometry, Fluorescence, Leukemia, Basophilic, Acute, Biochemistry, chemistry, Biophysics, Liberation, Oligomycins, gamma-Globulins, Intracellular, medicine.drug |
| الوصف: | Release of calcium from intracellular stores of rat basophilic leukemia cells was monitored using the fluorescent probe chlortetracycline. The ability of chlortetracycline to indicate release from intracellular calcium stores was initially validated. The decrease of chlortetracycline fluorescence upon antigen-stimulation was not the result of secretion of granule-associated dye or of changes in the properties of the membranes. The chlortetracycline fluorescence signal was not influenced by Ca2+ influx across the plasma membrane. Results obtained from these chlortetracycline fluorescence measurements corresponded well with 45Ca efflux data, an indirect measurement of release of calcium from stores. Chlortetracycline was used to examine the rate of antigen-induced release of calcium from stores, the depletion of intracellular calcium stores by EGTA, and the relationship between the antigen-stimulated release of stored calcium and exocytosis. Chlortetracycline was shown to be a useful qualitative indicator for the release of intracellular calcium with a relatively rapid response time. |
| تدمد: | 1097-4652 0021-9541 |
| DOI: | 10.1002/jcp.1041420111 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1aa6aa3ab643134e0ea22f509174e36c https://doi.org/10.1002/jcp.1041420111 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....1aa6aa3ab643134e0ea22f509174e36c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10974652 00219541 |
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| DOI: | 10.1002/jcp.1041420111 |