Ribosome engineering reveals the importance of 5S rRNA autonomy for ribosome assembly
| العنوان: | Ribosome engineering reveals the importance of 5S rRNA autonomy for ribosome assembly |
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| المؤلفون: | Anna B. Loveland, Amira Kefi, Nora Vázquez-Laslop, Kaspar Reier, Shijie Huang, Teresa Szal, Jaanus Remme, Nikolay A. Aleksashin, Andrei A. Korostelev, Dorota Klepacki, Alexander S. Mankin, Luc Jaeger |
| المصدر: | Nature Communications, Vol 11, Iss 1, Pp 1-13 (2020) Nature Communications |
| بيانات النشر: | University of Illinois at Chicago, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Ribosomal Proteins, 0301 basic medicine, Peptidyl transferase, Science, General Physics and Astronomy, Ribosome biogenesis, Ribosome Subunits, Large, Bacterial, Biology, Article, General Biochemistry, Genetics and Molecular Biology, Ribosome assembly, 03 medical and health sciences, 0302 clinical medicine, Ribosomal protein, 23S ribosomal RNA, Catalytic Domain, Escherichia coli, lcsh:Science, 50S, Uncategorized, Multidisciplinary, Escherichia coli Proteins, Cryoelectron Microscopy, RNA, Ribosomal, 5S, Translation (biology), General Chemistry, Ribosomal RNA, Ribosome, Cell biology, DNA-Binding Proteins, RNA, Bacterial, RNA, Ribosomal, 23S, Rec A Recombinases, 030104 developmental biology, Gene Expression Regulation, Mutation, Peptidyl Transferases, biology.protein, RNA, Nucleic Acid Conformation, lcsh:Q, Genetic Engineering, Ribosomes, 030217 neurology & neurosurgery |
| الوصف: | 5S rRNA is an indispensable component of cytoplasmic ribosomes in all species. The functions of 5S rRNA and the reasons for its evolutionary preservation as an independent molecule remain unclear. Here we used ribosome engineering to investigate whether 5S rRNA autonomy is critical for ribosome function and cell survival. By linking circularly permutated 5S rRNA with 23S rRNA we generated a bacterial strain devoid of free 5S rRNA. Viability of the engineered cells demonstrates that autonomous 5S rRNA is dispensable for cell growth under standard conditions and is unlikely to have essential functions outside the ribosome. The fully assembled ribosomes carrying 23S-5S rRNA are highly active in translation. However, the engineered cells accumulate aberrant 50S subunits unable to form stable 70S ribosomes. Cryo-EM analysis revealed a malformed peptidyl transferase center in the misassembled 50S subunits. Our results argue that the autonomy of 5S rRNA is preserved due to its role in ribosome biogenesis. Ribosomes of all organisms have retained 5S rRNA as an autonomous rRNA species. Here the authors engineer a bacterial strain with ribosomes that do not have free 5S rRNA, and carry structural analyses that suggest the evolutionary preservation of 5S rRNA as an independent molecule is based on its role in the dynamic process of ribosome biogenesis. |
| DOI: | 10.25417/uic.14498151 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::19a29b7ff3dade09fd7d94c1924d52bb |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....19a29b7ff3dade09fd7d94c1924d52bb |
| قاعدة البيانات: | OpenAIRE |
| DOI: | 10.25417/uic.14498151 |
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