Identification of gene expression signature in estrogen receptor positive breast carcinoma
| العنوان: | Identification of gene expression signature in estrogen receptor positive breast carcinoma |
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| المؤلفون: | Bhaskaran Muthuvelan, Debarshi Chakrabarti, N. Saravanan, Ravishankar, Arun Balakrishnan, Hemanth Raj, Muralidhara Padigaru, Arvind Thakkar |
| المصدر: | Biomarkers in Cancer Biomarkers in Cancer, Vol 2010, Iss 2, Pp 1-15 (2010) Biomarkers in Cancer, Vol 2 (2010) |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Microbiology (medical), Hormone response element, Candidate gene, business.industry, Immunology, Estrogen receptor, medicine.disease, Bioinformatics, lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens, lcsh:RC254-282, estrogen response element, Breast cancer, breast cancer, Gene expression, medicine, Cancer research, gene expression, Immunology and Allergy, E2F1, business, Breast carcinoma, Transcription factor, Original Research, estrogen receptor |
| الوصف: | A significant group of patient with estrogen receptor (ER) α positive breast tumors fails to appreciably respond to endocrine therapy. An increased understanding of the molecular basis of estrogen-mediated signal transduction and resultant gene expression may lead to novel strategies for treating breast cancer. In this study, we sought to identify the dysregulated genes in breast tumors related to ERα status. Microarray analyses of 31 tumor samples showed 108 genes differentially expressed in ERα (+) and ERα (–) primary breast tumors. Further analyses of gene lists indicated that a significant number of dysregulated genes were involved in mRNA transcription and cellular differentiation. The majority of these genes were found to have promoter-binding sites for E74-like factor 5 (ELF5; 54.6% genes), E2F transcription factor 1 (E2F1; 22.2% genes), and nuclear transcription factor Y alpha (NFYA; 32.4% genes). Six candidate genes ( NTN4, SLC7A8, MLPH, ENPP1, LAMB2, and PLAT) with differential expression were selected for further validation studies using RT-qPCR (76 clinical specimen) and immunohistochemistry (48 clinical specimen). Our studies indicate significant overexpression of all the six genes in ERα (+) breast tumors as compared to ERα (–) breast tumors. In vitro studies using T-47D breast cancer cell line confirmed the estrogen dependant expression of four of the above six genes ( SLC7A8, ENPP1, LAMB2, and PLAT). Collectively, our study provides further insights into the molecular basis of estrogen-dependent breast cancer and identifies “candidate biomarkers” that could be useful for predicting endocrine responsiveness. |
| تدمد: | 1179-299X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::195b0b7fa4720225c3cacc5dfa004dd9 https://pubmed.ncbi.nlm.nih.gov/24179381 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....195b0b7fa4720225c3cacc5dfa004dd9 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1179299X |
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