In Vitro Conservation of Sweet Potato Genotypes
العنوان: | In Vitro Conservation of Sweet Potato Genotypes |
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المؤلفون: | Maria Clezia dos Santos, Fernanda Ferreira Tavares, Arie Fitzgerald Blank, Aléa Dayane Dantas de Santana, Maria de Fátima Arrigoni-Blank, Thays Saynara Alves Menezes |
المصدر: | Repositório Institucional da UFS Universidade Federal de Sergipe (UFS) instacron:UFS The Scientific World Journal, Vol 2014 (2014) The Scientific World Journal |
بيانات النشر: | Hindawi Publishing Corporation, 2014. |
سنة النشر: | 2014 |
مصطلحات موضوعية: | Genotype, Article Subject, Every Three Months, Genótipo, Preservation, Biological, Genotypes, lcsh:Medicine, Biology, lcsh:Technology, General Biochemistry, Genetics and Molecular Biology, Batata-doce, Murashige and Skoog medium, In vitro, Botany, Ipomoea batatas, lcsh:Science, General Environmental Science, lcsh:T, lcsh:R, General Medicine, Slow growth, Horticulture, Shoot, lcsh:Q, Sweet Potato, Research Article |
الوصف: | The aim of this study was to develop a protocol for thein vitroconservation of sweet potato genotypes using the slow growth technique. The first experiment was conducted in a4×5×2factorial scheme, testing four genotypes (IPB-007, IPB-052, IPB-072, and IPB-137), five concentrations of abscisic acid (ABA) (0.0, 1.0, 2.0, 4.0, and 8.0 mg·L−1), and two temperatures (18 and 25°C). The second experiment was conducted in a4×3×3factorial scheme at 18°C, testing four genotypes (IPB-007, IPB-052, IPB-072, and IPB-137), three variations of MS salts (50, 75, and 100%), and three concentrations of sucrose (10, 20, and 30 g·L−1). Every three months, we evaluated the survival (%), shoot height, and shoot viability.In vitroconservation of the sweet potato genotypes IPB-052 and IPB-007 was obtained over three and six months, respectively, using MS medium plus 2.0 mg·L−1of ABA at either 18 or 25°C. Genotypes IPB-072 and IPB-137 can be kept for three and six months, respectively, in MS medium without ABA at 18°C. It is possible to store IPB-052 and IPB-072 for six months and IPB-007 and IPB-137 for nine months using 30 g·L−1of sucrose and 50% MS salts. |
وصف الملف: | text/xhtml |
اللغة: | English |
URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::19338d642a2953eb7c9d7429e3700a9c https://ri.ufs.br/handle/riufs/1579 |
Rights: | OPEN |
رقم الانضمام: | edsair.doi.dedup.....19338d642a2953eb7c9d7429e3700a9c |
قاعدة البيانات: | OpenAIRE |
الوصف غير متاح. |