A case of convergent-gene interference in the budding yeast knockout library causing chromosome instability
| العنوان: | A case of convergent-gene interference in the budding yeast knockout library causing chromosome instability |
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| المؤلفون: | Rong Li, Jin Zhu, Molly Gordon, Victoria Qu |
| المصدر: | G3: Genes|Genomes|Genetics G3: Genes, Genomes, Genetics, Vol 11, Iss 5 (2021) |
| بيانات النشر: | Oxford University Press (OUP), 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | AcademicSubjects/SCI01140, AcademicSubjects/SCI00010, YKO collection, convergent gene pairs, Mutant, Mitosis, Saccharomyces cerevisiae, QH426-470, Biology, AcademicSubjects/SCI01180, Chromosome segregation, 03 medical and health sciences, 0302 clinical medicine, Chromosomal Instability, Chromosome Segregation, Chromosome instability, Genetics, Humans, Kinetochores, Molecular Biology, Gene, SNO1, Genetics (clinical), 030304 developmental biology, Investigation, 0303 health sciences, Kinetochore, CTF13, Yeast, Terminator (genetics), Saccharomycetales, AcademicSubjects/SCI00960, chromosome instability, 030217 neurology & neurosurgery |
| الوصف: | To maintain genome stability, organisms depend on faithful chromosome segregation, a process affected by diverse genetic pathways, some of which are not directly linked to mitosis. In this study, we set out to explore one such pathway represented by an undercharacterized gene, SNO1, identified previously in screens of the yeast knockout (YKO) library for mitotic fidelity genes. We found that the causative factor increasing mitotic error rate in the sno1Δ mutant is not loss of the Sno1 protein, but rather perturbation to the mRNA of the neighboring convergent gene, CTF13, encoding an essential component for forming the yeast kinetochore. This is caused by a combination of the Kanamycin resistance gene and the transcriptional terminator used in the YKO library affecting the CTF13 mRNA level and quality . We further provide a list of gene pairs potentially subjected to this artifact, which may be useful for accurate phenotypic interpretation of YKO mutants. |
| تدمد: | 2160-1836 |
| DOI: | 10.1093/g3journal/jkab084 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::17c804577e05bdccf2ee6359a19c2d5e https://doi.org/10.1093/g3journal/jkab084 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....17c804577e05bdccf2ee6359a19c2d5e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 21601836 |
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| DOI: | 10.1093/g3journal/jkab084 |