Difference in the Vitreal Protein Profiles of Patients with Proliferative Diabetic Retinopathy with and without Intravitreal Conbercept Injection
| العنوان: | Difference in the Vitreal Protein Profiles of Patients with Proliferative Diabetic Retinopathy with and without Intravitreal Conbercept Injection |
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| المؤلفون: | Minjie Zhao, Dandan Zhu, Xinping She, Zhi Zheng, Yanan Hu, Jingjing Yu, Chen Zou, Yunzhi Wang |
| المصدر: | Journal of Ophthalmology, Vol 2018 (2018) Journal of Ophthalmology |
| بيانات النشر: | Hindawi Limited, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Article Subject, Apolipoprotein B, Inflammation, Pharmacology, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Platelet degranulation, lcsh:Ophthalmology, medicine, Platelet, biology, business.industry, Diabetic retinopathy, medicine.disease, eye diseases, Complement system, Vascular endothelial growth factor, Ophthalmology, 030104 developmental biology, chemistry, lcsh:RE1-994, 030221 ophthalmology & optometry, biology.protein, cardiovascular system, sense organs, medicine.symptom, Ceruloplasmin, business, Research Article |
| الوصف: | Purpose. To examine the difference in the vitreal protein profiles of patients with proliferative diabetic retinopathy (PDR) with and without preoperative intravitreal conbercept (IVC) treatment.Methods. Liquid chromatography-tandem mass spectrometry- (LC-MS/MS-) based proteomic methods were used to determine the protein profiles of the vitreous humor in patients with PDR treated with (IVC group;n=9) and without (PDR group;n=8) preoperative IVC. Gene ontology (GO) annotation and REACTOME pathway analysis were obtained to overview differentially expressed proteins between each group. Intravitreal levels of apolipoprotein A-II (APOA2) and ceruloplasmin (CP) were measured using enzyme-linked immunosorbent assays.Results. 307 proteins were expressed differentially between PDR and IVC groups, including 218 proteins downregulated in response to IVC. The most notable GO annotations in level 3 and REACTOME pathways describing the differentially expressed proteins were “innate immune response” and “platelet degranulation.” The intravitreal levels of APOA2 and CP were lower in the IVC group than in the PDR group (p<0.01).Conclusions. In addition to decreasing the intravitreal vascular endothelial growth factor level, IVC may alter the vitreal protein profile in patients with PDR, with the differentially regulated proteins involved in the immune response, platelet degranulation, complement activation, and inflammation. |
| وصف الملف: | text/xhtml |
| اللغة: | English |
| تدمد: | 2090-0058 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1747da5e6c598d46bc83420047ad5c77 https://doaj.org/article/d2bb8cef180642be8558997bc140bf0e |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....1747da5e6c598d46bc83420047ad5c77 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20900058 |
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