التفاصيل البيبلوغرافية
| العنوان: |
Cationic liposomes formulated with DMPC and a gemini surfactant traverse the cell membrane without causing a significant bio-damage |
| المؤلفون: |
Giuseppe Gigli, Cecilia Bombelli, F. Bordi, E. Stefanutti, Giovanna Mancini, F. Papacci, Simona Sennato, Ilenia Viola, Gianfranco Risuleo, Adalberto Bonincontro |
| المصدر: |
Biochimica et biophysica acta. Biomembranes 1838 (2014): 2646–2655. doi:10.1016/j.bbamem.2014.05.026
info:cnr-pdr/source/autori:Stefanutti E.[ 1 ]; Papacci F.[ 2 ]; Sennato S.[ 1,3 ]; Bombelli C.[ 4,5 ]; Viola I.[ 6 ] ; Bonincontro A.[ 1 ]; Bordi F.[ 1,3,7 ]; Mancini G.[ 4,5 ] ; Gigli G.[ 6,1,8 ] ; Risuleo G.[ 2 ]/titolo:Cationic liposomes formulated with DMPC and a gemini surfactant traverse the cell membrane without causing a significant bio-damage/doi:10.1016%2Fj.bbamem.2014.05.026/rivista:Biochimica et biophysica acta. Biomembranes/anno:2014/pagina_da:2646/pagina_a:2655/intervallo_pagine:2646–2655/volume:1838 |
| بيانات النشر: |
Elsevier BV, 2014. |
| سنة النشر: |
2014 |
| مصطلحات موضوعية: |
Cytoplasm, Biophysics, liposome penetration, Nanotechnology, Endocytosis, Membrane Fusion, Biochemistry, Cell Line, law.invention, Cell membrane, Mice, Surface-Active Agents, Pulmonary surfactant, Confocal microscopy, law, medicine, Animals, Cationic liposome, Liposome, Chemistry, Cell Membrane, Cell Biology, Quaternary Ammonium Compounds, biological effect, Membrane, medicine.anatomical_structure, phospholipid liposome, cell surface roughness, Liposomes, cationic gemini surfactant, Dimyristoylphosphatidylcholine |
| الوصف: |
Cationic liposomes have been intensively studied both in basic and applied research because of their promising potential as non-viral molecular vehicles. This work was aimed to gain more information on the interactions between the plasmamembrane and liposomes formed by a natural phospholipid and a cationic surfactant of the gemini family. The present work was conducted with the synergistic use of diverse experimental approaches: electro-rotation measurements, atomic force microscopy, ?-potential measurements, laser scanning confocal microscopy and biomolecular/cellular techniques. Electro-rotation measurements pointed out that the interaction of cationic liposomes with the cell membrane alters significantly its dielectric and geometric parameters. This alteration, being accompanied by significant changes of the membrane surface roughness as measured by atomic force microscopy, suggests that the interaction with the liposomes causes locally substantial modifications to the structure and morphology of the cell membrane. However, the results of electrophoretic mobility (?-potential) experiments show that upon the interaction the electric charge exposed on the cell surface does not vary significantly, pointing out that the simple adhesion on the cell surface of the cationic liposomes or their fusion with the membrane is to be ruled out. As a matter of fact, confocal microscopy images directly demonstrated the penetration of the liposomes inside the cell and their diffusion within the cytoplasm. Electro-rotation experiments performed in the presence of endocytosis inhibitors suggest that the internalization is mediated by, at least, one specific pathway. Noteworthy, the liposome uptake by the cell does not cause a significant biological damage. © 2014 Elsevier B.V. |
| تدمد: |
0005-2736 |
| DOI: |
10.1016/j.bbamem.2014.05.026 |
| URL الوصول: |
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::17171a6cc7e3f07561d72614f2b00f89 |
| Rights: |
OPEN |
| رقم الانضمام: |
edsair.doi.dedup.....17171a6cc7e3f07561d72614f2b00f89 |
| قاعدة البيانات: |
OpenAIRE |