Dihydropyrrolopyrazol-6-one MCHR1 antagonists for the treatment of obesity: Insights on in vivo efficacy from a novel FLIPR assay setup

التفاصيل البيبلوغرافية
العنوان: Dihydropyrrolopyrazol-6-one MCHR1 antagonists for the treatment of obesity: Insights on in vivo efficacy from a novel FLIPR assay setup
المؤلفون: Neil Flynn, Mary Jane Cullen, Hongwei Zhang, Wei Wang, Susan Glick, Lisa Zhang, Jeffrey A. Robl, Pratik Devasthale, Andres S. Hernandez, Christian Caporuscio, Brian J. Murphy, Suzanne Rooney, Ning Huang, Mary Ann Pelleymounter, Kenneth W. Rohrbach, Kishore Renduchintala, Anthony V. Azzara, Helen E. Godonis, Yi-Xin Li, Radhakrishnan Sridhar, Michael Thomas, Susan Harvey, Paul Stetsko, Mary F. Grubb, Chris Freeden, Fang Moore, Christine Huang, James Devenny, Daniel Longhi, William N. Washburn
المصدر: Bioorganic & Medicinal Chemistry Letters. 25:2793-2799
بيانات النشر: Elsevier BV, 2015.
سنة النشر: 2015
مصطلحات موضوعية: Chemistry, Organic Chemistry, Clinical Biochemistry, Pharmaceutical Science, Pharmacology, Biochemistry, In vitro, Rats, Rats, Sprague-Dawley, Structure-Activity Relationship, In vivo, Weight Loss, Drug Discovery, Animals, Humans, Pyrazoles, Molecular Medicine, Anti-Obesity Agents, Obesity, Receptors, Somatostatin, Molecular Biology, Incubation, Half-Life, Protein Binding
الوصف: Our investigation of the structure–activity and structure–liability relationships for dihydropyrrolopyrazol-6-one MCHR1 antagonists revealed that off-rate characteristics, inferred from potencies in a FLIPR assay following a 2 h incubation, can impact in vivo efficacy. The in vitro and exposure profiles of dihydropyrrolopyrazol-6-ones 1b and 1e were comparable to that of the thienopyrimidinone counterparts 41 and 43 except for a much faster MCHR1 apparent off-rate. The greatly diminished dihydropyrrolopyrazol-6-one anti-obesity response may be the consequence of this rapid off-rate.
تدمد: 0960-894X
DOI: 10.1016/j.bmcl.2015.05.008
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::17121b519c7b9c469d07059362c3d928
https://doi.org/10.1016/j.bmcl.2015.05.008
Rights: CLOSED
رقم الانضمام: edsair.doi.dedup.....17121b519c7b9c469d07059362c3d928
قاعدة البيانات: OpenAIRE
الوصف
تدمد:0960894X
DOI:10.1016/j.bmcl.2015.05.008