Linker Nups connect the nuclear pore complex inner ring with the outer ring and transport channel
| العنوان: | Linker Nups connect the nuclear pore complex inner ring with the outer ring and transport channel |
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| المؤلفون: | Roman Teimer, Ed Hurt, Stefan Amlacher, Ruth Kunze, Jessica Fischer |
| المصدر: | Nature Structural & Molecular Biology. 22:774-781 |
| بيانات النشر: | Springer Science and Business Media LLC, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Models, Molecular, Blotting, Western, Protomer, Chaetomium, Ring (chemistry), Chromatography, Affinity, Structural Biology, Yeasts, Escherichia coli, otorhinolaryngologic diseases, medicine, Cloning, Molecular, Nuclear pore, Binding site, Molecular Biology, Chemistry, Nuclear Pore Complex Proteins, stomatognathic diseases, medicine.anatomical_structure, Cytoplasm, Chromatography, Gel, Nuclear Pore, Biophysics, Nucleoporin, Nucleus, Linker, Plasmids |
| الوصف: | Nuclear pore complexes (NPCs) mediate transport between the nucleus and cytoplasm. NPCs are composed of ∼30 nucleoporins (Nups), most of which are organized in stable subcomplexes. How these modules are interconnected within the large NPC framework has been unknown. Here we show a mechanism of how supercomplexes can form between NPC modules. The Nup192 inner-pore-ring complex serves as a seed to which the Nup82 outer-ring complex and Nsp1 channel complex are tethered. The linkage between these subcomplexes is generated by short sequences within linker Nups. The conserved Nup145N is the most versatile connector of NPC modules because it has three discrete binding sites for Nup192, Nup170 and Nup82. We assembled a large part of a Chaetomium thermophilum NPC protomer in vitro, providing a step forward toward the reconstitution of the entire NPC. |
| تدمد: | 1545-9985 1545-9993 |
| DOI: | 10.1038/nsmb.3084 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::166938de1b0c5b4637980b26a70b1b2f https://doi.org/10.1038/nsmb.3084 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....166938de1b0c5b4637980b26a70b1b2f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15459985 15459993 |
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| DOI: | 10.1038/nsmb.3084 |