Akt and SGK protein kinases are required for efficient feeding by macropinocytosis
| العنوان: | Akt and SGK protein kinases are required for efficient feeding by macropinocytosis |
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| المؤلفون: | Sew-Yeu Peak-Chew, Thomas D. Williams, Robert R. Kay, Peggy Paschke |
| المصدر: | Journal of Cell Science. |
| بيانات النشر: | The Company of Biologists, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0303 health sciences, Phosphoinositide 3-kinase, biology, Protozoan Proteins, macromolecular substances, Cell Biology, Protein Serine-Threonine Kinases, Endocytosis, biology.organism_classification, Dictyostelium, Article, Dictyostelium discoideum, Cell biology, 03 medical and health sciences, 0302 clinical medicine, biology.protein, Pinocytosis, Macropinosome, Protein kinase A, Proto-Oncogene Proteins c-akt, Protein kinase B, 030217 neurology & neurosurgery, PI3K/AKT/mTOR pathway, 030304 developmental biology |
| الوصف: | Macropinocytosis is an actin-driven process of large-scale and non-specific fluid uptake used for feeding by some cancer cells and the macropinocytosis model organism Dictyostelium discoideum. In Dictyostelium, macropinocytic cups are organized by ‘macropinocytic patches’ in the plasma membrane. These contain activated Ras, Rac and phospholipid PIP3, and direct actin polymerization to their periphery. We show that a Dictyostelium Akt (PkbA) and an SGK (PkbR1) protein kinase act downstream of PIP3 and, together, are nearly essential for fluid uptake. This pathway enables the formation of larger macropinocytic patches and macropinosomes, thereby dramatically increasing fluid uptake. Through phosphoproteomics, we identify a RhoGAP, GacG, as a PkbA and PkbR1 target, and show that it is required for efficient macropinocytosis and expansion of macropinocytic patches. The function of Akt and SGK in cell feeding through control of macropinosome size has implications for cancer cell biology. |
| تدمد: | 1477-9137 0021-9533 |
| DOI: | 10.1242/jcs.224998 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::161875d89183de0a1ed6833a7060eb96 https://doi.org/10.1242/jcs.224998 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....161875d89183de0a1ed6833a7060eb96 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14779137 00219533 |
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| DOI: | 10.1242/jcs.224998 |