Direct conversion of pig fibroblasts to chondrocyte-like cells by c-Myc
| العنوان: | Direct conversion of pig fibroblasts to chondrocyte-like cells by c-Myc |
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| المؤلفون: | Weiwang Gu, Wen-Tao Zhao, Yan Sun, Sheng-Chun Wang, Jun-Shuang Jia, Jing Li, Xiao-Lin Lin, Jie Yang, Dong Xiao, Jun-Wen Shi, Hong-Fen Shen, Wei Liu, Ting-Ting Zhang |
| المصدر: | Cell Death Discovery, Vol 5, Iss 1, Pp 1-15 (2019) Cell Death Discovery |
| بيانات النشر: | Nature Publishing Group, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Cancer Research, Somatic cell, Immunology, Cell morphology, lcsh:RC254-282, Article, Chondrocyte, 03 medical and health sciences, Cellular and Molecular Neuroscience, 0302 clinical medicine, In vivo, medicine, lcsh:QH573-671, Chemistry, lcsh:Cytology, Cell Biology, lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens, Embryonic stem cell, Molecular biology, In vitro, Staining, 030104 developmental biology, medicine.anatomical_structure, 030220 oncology & carcinogenesis, Ectopic expression |
| الوصف: | Unexpectedly, we found that c-Myc-expressing porcine embryonic fibroblasts (PEFs) subcutaneously implanted into nude mice formed cartilage-like tissues in vivo, while previous studies revealed the direct conversion of mouse and human somatic cells into chondrocytes by the combined use of several defined factors, including c-Myc, which prompted us to explore whether PEFs can be reprogrammed to become pig induced chondrocyte-like cells (piCLCs) via ectopic expression of c-Myc alone. In this study, c-Myc-expressing PEFs, designated piCLCs, which exhibited a significantly enhanced proliferation ability in vitro, displayed a chondrogenic phenotypes in vitro, as shown by the cell morphology, toluidine blue staining, alcian blue staining and chondrocyte marker gene expression. Additionally, piCLCs with a polygonal chondrocyte-like morphology were readily and efficiently converted from PEFs by enforced c-Myc expression within 10 days, while piCLCs maintained the chondrocytic phenotype and normal karyotype during long-term subculture. piCLC-derived single clones with a chondrogenic phenotype in vitro exhibited homogeneity in cell morphology and staining intensity compared with mixed piCLCs. Although the mixtures of cartilaginous tissues and tumorous tissues accounted for ~12% (6/51) of all xenografts (51), piCLCs generated stable, homogenous, hyaline cartilage-like tissues without tumour formation at 45 out of the 51 injected sites when subcutaneously injected into nude mice. The hyaline cartilage-like tissues remained for at least 16 weeks. Taken together, these findings demonstrate for the first time the direct induction of chondrocyte-like cells from PEFs with only c-Myc. |
| اللغة: | English |
| تدمد: | 2058-7716 |
| DOI: | 10.1038/s41420-018-0136-4 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::149f7e241749b4bf29447a2e1fa4c0a6 http://link.springer.com/article/10.1038/s41420-018-0136-4 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....149f7e241749b4bf29447a2e1fa4c0a6 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20587716 |
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| DOI: | 10.1038/s41420-018-0136-4 |