Construction and characterization of a double mutant of Enterococcus faecalis that does not produce biogenic amines
| العنوان: | Construction and characterization of a double mutant of Enterococcus faecalis that does not produce biogenic amines |
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| المؤلفون: | Oscar P. Kuipers, Miguel A. Alvarez, Begoña Redruello, M. Cruz Martín, Beatriz del Rio, Marta Pérez, Maria J. Fernandez, Marina Calles-Enríquez, Jan Kok, Anne de Jong, Victor Ladero |
| المساهمون: | Ministerio de Economía y Competitividad (España), Principado de Asturias, European Commission, Río Lagar, Beatriz del [0000-0001-8107-1975], Martín, M. Cruz [0000-0003-1975-6775], Ladero Losada, Víctor Manuel [0000-0002-7613-3745], Álvarez González, Miguel Ángel [0000-0001-9607-7480], Molecular Genetics, Río Lagar, Beatriz del, Martín, M. Cruz, Ladero Losada, Víctor Manuel, Álvarez González, Miguel Ángel |
| المصدر: | Digital.CSIC. Repositorio Institucional del CSIC instname Scientific Reports, 9(1):16881. Nature Publishing Group Scientific Reports Scientific Reports, Vol 9, Iss 1, Pp 1-9 (2019) |
| بيانات النشر: | Springer Nature, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Molecular biology, 030106 microbiology, TYROSINE DECARBOXYLATION PATHWAY, lcsh:Medicine, LACTIC-ACID BACTERIA, Enterococcus faecalis, Article, Bacterial Adhesion, Applied microbiology, 03 medical and health sciences, chemistry.chemical_compound, Biosynthesis, TYRAMINE, Humans, BIOSYNTHESIS, lcsh:Science, Homologous Recombination, LACTOCOCCUS-LACTIS, Fermentation in food processing, Gene, Multidisciplinary, biology, Chemistry, LOW PH, STRAINS, lcsh:R, Lactococcus lactis, Gene Expression Regulation, Bacterial, Tyramine, Chromosomes, Bacterial, Hydrogen-Ion Concentration, biology.organism_classification, PUTRESCINE, EVOLUTION, 030104 developmental biology, Biochemistry, Biofilms, Multigene Family, Putrescine, Food Microbiology, lcsh:Q, Caco-2 Cells, Homologous recombination, Genetic Engineering, Transcriptome, Gene Deletion, GENERATION |
| الوصف: | Enterococcus faecalis is a lactic acid bacterium characterized by its tolerance of very diverse environmental conditions, a property that allows it to colonize many different habitats. This species can be found in food products, especially in fermented foods where it plays an important role as a biopreservative and influences the development of organoleptic characteristics. However, E. faecalis also produces the biogenic amines tyramine and putrescine. The consumption of food with high concentrations of these compounds can cause health problems. The present work reports the construction, via homologous recombination, of a double mutant of E. faecalis in which the clusters involved in tyramine and putrescine synthesis (which are located in different regions of the chromosome) are no longer present. Analyses showed the double mutant to grow and adhere to intestinal cells normally, and that the elimination of genes involved in the production of tyramine and putrescine has no effect on the expression of other genes. Tis work was performed with the fnancial support of AEI under grant number AGL2016-78708-R, (AEI/FEDER, UE) and the Plan for Science, Technology and Innovation 2013–2017 of the Principality of Asturias, co-funded by FEDER (GRUPIN IDI/2018/000114). |
| وصف الملف: | application/pdf |
| تدمد: | 2045-2322 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::145de94e2812d241983f8c54bac12934 http://hdl.handle.net/10261/201803 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....145de94e2812d241983f8c54bac12934 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20452322 |
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