Dissecting a Role for Melanopsin in Behavioural Light Aversion Reveals a Response Independent of Conventional Photoreception
| العنوان: | Dissecting a Role for Melanopsin in Behavioural Light Aversion Reveals a Response Independent of Conventional Photoreception |
|---|---|
| المؤلفون: | J. M. Lawrence, Ahmad Ahmado, Ma'ayan Semo, Peter J. Coffey, Eriko Sugano, Anthony A. Vugler, Annette E. Allen, Hiroshi Tomita, Carlos Gias |
| المصدر: | PLoS ONE PLOS ONE, 5 (11), Article e15009. (2010) PLoS ONE, Vol 5, Iss 11, p e15009 (2010) |
| بيانات النشر: | Public Library of Science, 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Atropine, Mouse, Light, Visual System, Emotions, Channelrhodopsin, lcsh:Medicine, Social and Behavioral Sciences, Open field, Behavioral Neuroscience, Mice, Psychology, lcsh:Science, Visual Cortex, GNAT1, RETINAL GANGLION-CELLS, SUPERIOR COLLICULUS, MICE LACKING, PUPILLARY CONSTRICTION, OCULAR PHOTORECEPTORS, RETROSPLENIAL CORTEX, VISUAL-CORTEX, MOUSE MODEL, NON-CONE, NON-ROD, Mice, Knockout, Multidisciplinary, medicine.diagnostic_test, Anatomy, Animal Models, Immunohistochemistry, Sensory Systems, GTP-Binding Protein alpha Subunits, Rhodopsin, Regression Analysis, Genetic Engineering, Proto-Oncogene Proteins c-fos, Research Article, Biotechnology, Melanopsin, Mice, 129 Strain, Cyclic Nucleotide-Gated Cation Channels, Biology, Stimulus (physiology), Motor Activity, Retina, Model Organisms, Channelrhodopsins, medicine, Avoidance Learning, Electroretinography, Animals, Humans, Transducin, Behavior, Superior colliculus, lcsh:R, Rod Opsins, Parasympatholytics, Mice, Inbred C57BL, Kinetics, biology.protein, lcsh:Q, sense organs, Neuroscience, Transgenics |
| الوصف: | Melanopsin photoreception plays a vital role in irradiance detection for non-image forming responses to light. However, little is known about the involvement of melanopsin in emotional processing of luminance. When confronted with a gradient in light, organisms exhibit spatial movements relative to this stimulus. In rodents, behavioural light aversion (BLA) is a well-documented but poorly understood phenomenon during which animals attribute salience to light and remove themselves from it. Here, using genetically modified mice and an open field behavioural paradigm, we investigate the role of melanopsin in BLA. While wildtype (WT), melanopsin knockout (Opn4(-/-)) and rd/rd cl (melanopsin only (MO)) mice all exhibit BLA, our novel methodology reveals that isolated melanopsin photoreception produces a slow, potentiating response to light. In order to control for the involvement of pupillary constriction in BLA we eliminated this variable with topical atropine application. This manipulation enhanced BLA in WT and MO mice, but most remarkably, revealed light aversion in triple knockout (TKO) mice, lacking three elements deemed essential for conventional photoreception (Opn4(-/-) Gnat1(-/-) Cnga3(-/-)). Using a number of complementary strategies, we determined this response to be generated at the level of the retina. Our findings have significant implications for the understanding of how melanopsin signalling may modulate aversive responses to light in mice and humans. In addition, we also reveal a clear potential for light perception in TKO mice. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1932-6203 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1428e840e6b94f726699d83de727db51 http://europepmc.org/articles/PMC2993953 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....1428e840e6b94f726699d83de727db51 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
|---|