Author Correction: Development and performance of a targeted whole exome sequencing enrichment kit for the dog (Canis Familiaris Build 3.1)
| العنوان: | Author Correction: Development and performance of a targeted whole exome sequencing enrichment kit for the dog (Canis Familiaris Build 3.1) |
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| المؤلفون: | Filip Van Nieuwerburgh, Dieter Deforce, Valérie Bavegems, Sarah De Keulenaer, Frank Coopman, Bart J. G. Broeckx, Ellen De Meester, Geert E. C. Verhoeven |
| المصدر: | Scientific Reports, Vol 10, Iss 1, Pp 1-1 (2020) Scientific Reports |
| بيانات النشر: | Springer Science and Business Media LLC, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Multidisciplinary, biology, lcsh:R, High-Throughput Nucleotide Sequencing, Reproducibility of Results, lcsh:Medicine, Sequence Analysis, DNA, Computational biology, biology.organism_classification, Sensitivity and Specificity, Dogs, Canis, Animals, Exome, lcsh:Q, Author Correction, lcsh:Science, Exome sequencing |
| الوصف: | Whole exome sequencing is a technique that aims to selectively sequence all exons of protein-coding genes. A canine whole exome sequencing enrichment kit was designed based on the latest canine reference genome (build 3.1.72). Its performance was tested by sequencing 2 exome captures, each consisting of 4 pre-capture pooled, barcoded Illumina libraries on an Illumina HiSeq 2500. At an average sequencing depth of 102x, 83 to 86% of the target regions were completely sequenced with a minimum coverage of five and 90% of the reads mapped on the target regions. Additionally, it is shown that the reproducibility within and between captures is high and that pooling four samples per capture is a valid option. Overall, we have demonstrated the strong performance of this WES enrichment kit and are confident it will be a valuable tool in future disease association studies. |
| تدمد: | 2045-2322 |
| DOI: | 10.1038/s41598-020-62785-3 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1418b39faa68d783419c4b18fb4eaa4b https://doi.org/10.1038/s41598-020-62785-3 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....1418b39faa68d783419c4b18fb4eaa4b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20452322 |
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| DOI: | 10.1038/s41598-020-62785-3 |