Identification of valine/leucine/isoleucine and threonine/alanine/glycine proton-spin systems of Escherichia coli adenylate kinase by selective deuteration and selective protonation
| العنوان: | Identification of valine/leucine/isoleucine and threonine/alanine/glycine proton-spin systems of Escherichia coli adenylate kinase by selective deuteration and selective protonation |
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| المؤلفون: | Birgit Brandmeier, Paul Rösch, Imke Bock-Möbius, Marie-Therese Dauvergne, Alfred Wittinghofer, Reuben Leberman, Herbert Zimmermann, Sabine Zimmermann, Martin Brune |
| المصدر: | Biochemical Journal. 273:311-316 |
| بيانات النشر: | Portland Press Ltd., 1991. |
| سنة النشر: | 1991 |
| مصطلحات موضوعية: | Alanine, Magnetic Resonance Spectroscopy, Stereochemistry, Chemistry, Adenylate Kinase, Adenylate kinase, Phenylalanine, Cell Biology, Deuterium, medicine.disease_cause, Biochemistry, Valine, Escherichia coli, medicine, heterocyclic compounds, Amino Acids, Threonine, Leucine, Isoleucine, Molecular Biology, Research Article |
| الوصف: | Adenylate kinase from two types of Escherichia coli strains, a wild-type and a leucine-auxotrophic strain, was purified. On the one hand, growing the leucine-auxotrophic bacteria on a medium containing deuterated leucine yielded E. coli adenylate kinase with all leucine residues deuterated. On the other hand, by growing the wild-type bacteria on deuterated medium with phenylalanine, threonine and isoleucine present as protonated specimens, 80% randomly deuterated enzyme with protonated phenylalanine, threonine and isoleucine residues could be prepared. Use of these proteins enabled identification of the spin systems of these amino acid residues in the n.m.r. spectra of the protein. |
| تدمد: | 1470-8728 0264-6021 |
| DOI: | 10.1042/bj2730311 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::138e3e05957567bf5339e2941eef6b8c https://doi.org/10.1042/bj2730311 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....138e3e05957567bf5339e2941eef6b8c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14708728 02646021 |
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| DOI: | 10.1042/bj2730311 |