LC-MS vs. GC-MS, online extraction systems, advantages of technology for drug screening assays
| العنوان: | LC-MS vs. GC-MS, online extraction systems, advantages of technology for drug screening assays |
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| المؤلفون: | Pierre Marquet |
| المساهمون: | Pharmacologie des Immunosuppresseurs et de la Transplantation ( PIST ), Université de Limoges ( UNILIM ) -CHU Limoges-Génomique, Environnement, Immunité, Santé, Thérapeutique ( GEIST FR CNRS 3503 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ), Service de Pharmacologie, toxicologie et pharmacovigilance [CHU Limoges], CHU Limoges, Pharmacologie des Immunosuppresseurs et de la Transplantation (PIST), Université de Limoges (UNILIM)-CHU Limoges-Génomique, Environnement, Immunité, Santé, Thérapeutique (GEIST FR CNRS 3503)-Institut National de la Santé et de la Recherche Médicale (INSERM), Marquet, Pierre |
| المصدر: | Methods in Molecular Biology (Clifton then Totova) Methods in Molecular Biology (Clifton then Totova), Humana Press (Springer Imprint), 2012, 902, pp.15-27. 〈10.1007/978-1-61779-934-1_2〉 Methods in Molecular Biology Methods in Molecular Biology, Humana Press/Springer Imprint, 2012, 902, pp.15-27. ⟨10.1007/978-1-61779-934-1_2⟩ Methods in Molecular Biology ISBN: 9781617799334 |
| بيانات النشر: | HAL CCSD, 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | MESH : Drug-Related Side Effects and Adverse Reactions, Chromatography, Gas, MESH: High-Throughput Screening Assays, Drug-Related Side Effects and Adverse Reactions, MESH: Pharmaceutical Preparations, MESH: Substance Abuse Detection, Pharmacology, 010402 general chemistry, Mass spectrometry, MESH : High-Throughput Screening Assays, Online Systems, 01 natural sciences, Mass Spectrometry, MESH : Chromatography, Gas, MESH: Software, MESH : Chromatography, High Pressure Liquid, MESH : Pharmaceutical Preparations, MESH : Mass Spectrometry, MESH : Substance Abuse Detection, Liquid chromatography–mass spectrometry, High-Throughput Screening Assays, Sample preparation, MESH: Chromatography, High Pressure Liquid, Chromatography, High Pressure Liquid, MESH: Mass Spectrometry, Chromatography, Chemistry, 010401 analytical chemistry, MESH: Chromatography, Gas, [SDV.SP]Life Sciences [q-bio]/Pharmaceutical sciences, MESH: Drug-Related Side Effects and Adverse Reactions, MESH: Online Systems, 0104 chemical sciences, Substance Abuse Detection, [SDV.SP] Life Sciences [q-bio]/Pharmaceutical sciences, Time of flight, [ SDV.SP ] Life Sciences [q-bio]/Pharmaceutical sciences, Pharmaceutical Preparations, Mass spectrum, Gas chromatography, MESH : Software, Gas chromatography–mass spectrometry, MESH : Online Systems, Software |
| الوصف: | International audience; This chapter reviews recent applications of mass spectrometry to systematic toxicological analysis (STA), where extended lists of compounds of toxicological interest are screened, as well as to the general unknown screening (GUS), where all exogenous compounds present in a sample are tentatively detected and identified, without any preselection. Many recent improvements in sample preparation, chromatographic separation, gas chromatography-mass spectrometry, and above all liquid chromatography-mass spectrometry techniques are described, which are applicable or have been applied to STA and/or GUS, generally with promising results. These improvements come from miniaturization and automation of solid-phase extraction, turbulent-flow or ultrahigh-pressure liquid chromatography, linear ion traps, accurate (e.g., time of flight or orbital trap) mass spectrometry, as well as software refinements to alternate between different ionization modes or automatically interpret the results. It also shows that robust LC-MS/MS techniques already exist for STA or GUS, which are at least as efficient as the traditional techniques used in most toxicology laboratories, such as GC-MS or high-performance liquid chromatography with diode-array detection, as shown by three comparative studies. However, the major drawback of LC-MS/MS in the full-scan mode for STA or GUS is that it still lacks universal reference libraries due to insufficient reproducibility of LC-MS(/MS) mass spectra obtained with different instrument types. |
| اللغة: | English |
| ردمك: | 978-1-61779-933-4 |
| تدمد: | 1064-3745 1940-6029 |
| DOI: | 10.1007/978-1-61779-934-1_2〉 |
| DOI: | 10.1007/978-1-61779-934-1_2⟩ |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::130bf5441b7d988d0d0bdb6bf8d74e6c http://www.hal.inserm.fr/inserm-00925553 |
| رقم الانضمام: | edsair.doi.dedup.....130bf5441b7d988d0d0bdb6bf8d74e6c |
| قاعدة البيانات: | OpenAIRE |
| ردمك: | 9781617799334 |
|---|---|
| تدمد: | 10643745 19406029 |
| DOI: | 10.1007/978-1-61779-934-1_2〉 |