miR-927 has pro-viral effects during acute and persistent infection with dengue virus type 2 in C6/36 mosquito cells
| العنوان: | miR-927 has pro-viral effects during acute and persistent infection with dengue virus type 2 in C6/36 mosquito cells |
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| المؤلفون: | Martha Yocupicio-Monroy, Laurence A. Marchat, Rodolfo Gamaliel Avila-Bonilla, David Guillermo Pérez-Ishiwara, Doris Cerecedo-Mercado, Rosa M. del Angel, Juan Santiago Salas-Benito |
| المصدر: | Journal of General Virology. 101:825-839 |
| بيانات النشر: | Microbiology Society, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, 030106 microbiology, Genome, Viral, Dengue virus, Virus Replication, medicine.disease_cause, Communicable Diseases, Cell Line, Dengue fever, Dengue, 03 medical and health sciences, Downregulation and upregulation, Aedes, Virology, medicine, Animals, Luciferases, Defensin, Reporter gene, Innate immune system, biology, Dengue Virus, medicine.disease, biology.organism_classification, MicroRNAs, Flavivirus, 030104 developmental biology, Viral load |
| الوصف: | Dengue virus (DENV) is an important flavivirus that is transmitted to humans byAedesmosquitoes, where it can establish a persistent infection underlying vertical and horizontal transmission. However, the exact mechanism of persistent DENV infection is not well understood. Recently miR-927 was found to be upregulated in C6/36-HT cells at 57 weeks of persistent infection (C6-L57), suggesting its participation during this type of infection. The aim of this study was to determine the role of miR-927 during infection with DENV type 2. The results indicate an overexpression of miR-927 in C6-L57 cells and acutely infected cells according to the time of infection and the m.o.i. used. The downregulation of miR-927 in C6-L57 cells results in a reduction of both viral titre and viral genome copy number. The overexpression of miR-927 in C6-L40 and C6/36 cells infected at an m.o.i. of 0.1 causes an increase in both viral titre and viral genome copy number, suggesting a pro-viral activity of miR-927.In silicoprediction analysis reveals target mRNAs for miR-927 are implicated in post-translational modifications (SUMO), translation factors (eIF-2B), the innate immune system (NKIRAS), exocytosis (EXOC-2), endocytosis (APM1) and the cytoskeleton (FLN). The expression levels of FLN were the most affected by both miR-927 overexpression and inhibition, and FLN was determined to be a direct target of miR-927 by a dual-luciferase gene reporter assay. FLN has been associated with the regulation of the Toll pathway and either overexpression or downregulation of miR-927 resulted in expression changes of antimicrobial peptides (Cecropins A and G, and Defensin D) involved in the Toll pathway response. |
| تدمد: | 1465-2099 0022-1317 |
| DOI: | 10.1099/jgv.0.001441 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::12776e1d13bcf978b9df4208eefde5ab https://doi.org/10.1099/jgv.0.001441 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....12776e1d13bcf978b9df4208eefde5ab |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14652099 00221317 |
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| DOI: | 10.1099/jgv.0.001441 |