Expression of a plastid-targeted flavodoxin decreases chloroplast reactive oxygen species accumulation and delays senescence in aging tobacco leaves
| العنوان: | Expression of a plastid-targeted flavodoxin decreases chloroplast reactive oxygen species accumulation and delays senescence in aging tobacco leaves |
|---|---|
| المؤلفون: | Mohammad R. Hajirezaei, Vanesa B. Tognetti, Michael Melzer, Néstor Carrillo, Martín L. Mayta, Anabella F. Lodeyro, Juan José Guiamet |
| المصدر: | RepHipUNR (UNR) Universidad Nacional de Rosario instacron:UNR Frontiers in Plant Science, Vol 9 (2018) CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET Frontiers in Plant Science SEDICI (UNLP) Universidad Nacional de La Plata instacron:UNLP |
| بيانات النشر: | Frontiers Media, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0106 biological sciences, 0301 basic medicine, Senescence, Chloroplasts, Flavodoxin, Plant Science, lcsh:Plant culture, Photosynthesis, 01 natural sciences, Ciencias Biológicas, purl.org/becyt/ford/1 [https], 03 medical and health sciences, Auxin, Tobacco, lcsh:SB1-1110, Redox poise, Ciencias Agrarias, Plastid, purl.org/becyt/ford/1.6 [https], Carotenoid, Original Research, chemistry.chemical_classification, Reactive oxygen species, biology, Chemistry, fungi, Transgenic plants, food and beverages, Bioquímica y Biología Molecular, Cell biology, Chloroplast, 030104 developmental biology, biology.protein, CIENCIAS NATURALES Y EXACTAS, 010606 plant biology & botany |
| الوصف: | Leaf senescence is a concerted physiological process involving controlled degradation of cellular structures and reallocation of breakdown products to other plant organs. It is accompanied by increased production of reactive oxygen species (ROS) that are proposed to signal cell death, although both the origin and the precise role of ROS in the execution of this developmental program are still poorly understood. To investigate the contribution of chloroplast-associated ROS to natural leaf senescence, we used tobacco plants expressing a plastid-targeted flavodoxin, an electron shuttle flavoprotein present in prokaryotes and algae. When expressed in plants, flavodoxin specifically prevents ROS formation in chloroplasts during stress situations. Senescence symptoms were significantly mitigated in these transformants, with decreased accumulation of chloroplastic ROS and differential preservation of chlorophylls, carotenoids, protein contents, cell and chloroplast structures, membrane integrity and cell viability. Flavodoxin also improved maintenance of chlorophyll-protein complexes, photosynthetic electron flow, CO2 assimilation, central metabolic routes and levels of bioactive cytokinins and auxins in aging leaves. Delayed induction of senescence-associated genes indicates that the entire genetic program of senescence was affected by flavodoxin. The results suggest that ROS generated in chloroplasts are involved in the regulation of natural leaf senescence. Instituto de Fisiología Vegetal |
| وصف الملف: | application/pdf |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::121167f966eaf2f957d0be8e872018b9 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....121167f966eaf2f957d0be8e872018b9 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |