Altered Expression of MicroRNAs in the Bone Marrow of Multiple Myeloma Patients and their Relationship to Cytogenetic Aberrations

التفاصيل البيبلوغرافية
العنوان: Altered Expression of MicroRNAs in the Bone Marrow of Multiple Myeloma Patients and their Relationship to Cytogenetic Aberrations
المؤلفون: Maha M. Eid, Amany H. Abdelrahman, Ola M. Eid, Mahmoud M. Kamel, Mosaad M. El Gammal, Mohamed T.H. Sallam, Hanaa R.M. Attia, Mona H. Ibrahim
المصدر: Current pharmaceutical biotechnology. 21(13)
سنة النشر: 2019
مصطلحات موضوعية: 0301 basic medicine, Oncology, Adult, Male, medicine.medical_specialty, Pharmaceutical Science, Down-Regulation, Locus (genetics), Real-Time Polymerase Chain Reaction, Pathogenesis, 03 medical and health sciences, 0302 clinical medicine, Bone Marrow, Internal medicine, microRNA, Biomarkers, Tumor, Medicine, Humans, Epigenetics, Multiple myeloma, In Situ Hybridization, Fluorescence, Chromosome Aberrations, medicine.diagnostic_test, business.industry, Gene Expression Profiling, Cytogenetics, medicine.disease, Prognosis, Up-Regulation, MicroRNAs, 030104 developmental biology, medicine.anatomical_structure, 030220 oncology & carcinogenesis, Egypt, Female, Bone marrow, business, Multiple Myeloma, Biotechnology, Fluorescence in situ hybridization
الوصف: Background: Multiple Myeloma (MM) is a complex hematologic malignancy, driven by several genetic and epigenetic alterations. MiRNAs as biomarkers have become a rapidly growing research area in the last decade. Aim: The aim was to study the expression pattern of selected miRNAs and to explore the impact of cytogenetic aberrations in MM patients for therapeutic tools. Patients and Methods: Forty Egyptian adult patients were selected for the study with symptomatic newly diagnosed MM disease. Bone marrow samples were collected to investigate twelve miRNAs selected according to their relation to the most common cytogenetic aberrations with relevant prognostic value. The relative expression of the selected miRNAs was determined using a real-time PCR technique. Fluorescence In Situ Hybridization (FISH) technique was performed for cytogenetic analysis. Results: Eight miRNAs were down-regulated [miR-15a (p Conclusion: Deregulated miRNAs were identified and the association with 14q32 rearrangement and MM pathogenesis has been determined.
تدمد: 1873-4316
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::11753217379175a814987e14e312c988
https://pubmed.ncbi.nlm.nih.gov/32196445
رقم الانضمام: edsair.doi.dedup.....11753217379175a814987e14e312c988
قاعدة البيانات: OpenAIRE