Effect of cholesterol loaded cyclodextrin on semen cryopreservation of Aksaray Malakli shepherd dogs of different ages
| العنوان: | Effect of cholesterol loaded cyclodextrin on semen cryopreservation of Aksaray Malakli shepherd dogs of different ages |
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| المؤلفون: | Emre Durmaz, Koray Tekin, Kemal Tuna Olgac, Ongun Uysal, Beste Çil, Umut Taşdemir, Pürhan Barbaros Tuncer, Serhat Büyükleblebici, Muhammed Enes Inanç, Burak Yilmaz |
| المساهمون: | [Inanc, Muhammed Enes] Mehmet Akif Ersoy Univ, Fac Vet Med, Dept Reprod & Artificial Inseminat, Burdur, Turkey -- [Tekin, Koray -- Olgac, Kemal Tuna -- Yilmaz, Burak -- Cil, Beste -- Uysal, Ongun] Ankara Univ, Fac Vet Med, Dept Reprod & Artificial Inseminat, Ankara, Turkey -- [Tasdemir, Umut] Aksaray Univ, Tech Sci Vocat Sch, Aksaray, Turkey -- [Tuncer, Purhan Barbaros -- Buyukleblebici, Serhat] Mersin Univ, Tech Sci Vocat Sch, Mersin, Turkey -- [Durmaz, Emre] Gazi Univ, Fac Pharm, Dept Toxicol, Ankara, Turkey, Durmaz, Emre -- 0000-0003-2239-1762, Aksaray Teknik Bilimler Meslek Yüksekokulu |
| المصدر: | Animal reproduction science. 193 |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Male, Aging, Semen, Semen analysis, Cryopreservation, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Endocrinology, Animal science, Age, Cryoprotective Agents, Dogs, Food Animals, Freezing, medicine, Cyclodextrin, Animals, Acrosome, Malakli shepherd dog, chemistry.chemical_classification, Cyclodextrins, 030219 obstetrics & reproductive medicine, medicine.diagnostic_test, Chemistry, Cholesterol, 0402 animal and dairy science, Age Factors, 04 agricultural and veterinary sciences, General Medicine, 040201 dairy & animal science, Semen cryopreservation, Spermatozoa, Middle age, Semen Analysis, Sperm freezing, Sperm Motility, Animal Science and Zoology, Semen Preservation |
| الوصف: | WOS: 000433999000021 PubMed: 29699920 The objective of the study was to determine the effect of cholesterol-loaded cyclodextrin (CLC) on the quality parameters of semen from Aksaray Malakli Shepherd dogs of different age groups. Forty-eight male dogs were divided into 3 groupings according to their ages (young age (Y): = 7 years; n: 8). The sperm-rich portion of the ejaculate from each dog was divided into four aliquots and extended with either tris as a control (C) or tris loaded with 0.5, 1.0, and 1.5 mg/120 x 10(6) CLC as low (L), intermediate (I), and high (H) doses, respectively. Following equilibration for at least half an hour, the straws were frozen in nitrogen vapor and then stored in liquid nitrogen at least for 48 h. Later, the frozen straws were thawed in a water bath for spermatological evaluation. Significant differences were observed between different age groups in terms of the spermatological parameters (p < 0.05). The evidence suggests that increasing age is associated with poor in-vitro spermatological parameters and CLC was able to protect the acrosome integrity from cryo-damage during the freeze-thawing process. Better semen freezability characteristics were obtained at young ages, considering the overall parameters. Scientific and Technological Research Council of Turkey (TUBITAK) [1140636] This study was financed under the Project supported by the Scientific and Technological Research Council of Turkey (TUBITAK) (Project No:1140636). |
| وصف الملف: | application/pdf |
| تدمد: | 1873-2232 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::106acb1078bdd22c0a555091268412e2 https://pubmed.ncbi.nlm.nih.gov/29699920 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....106acb1078bdd22c0a555091268412e2 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18732232 |
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