Tissue fixation with phenol—formaldehyde for routine histopathology
| العنوان: | Tissue fixation with phenol—formaldehyde for routine histopathology |
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| المؤلفون: | D. Hopwood, G. R. Yeaman, W. Slidders |
| المصدر: | The Histochemical Journal. 21:228-234 |
| بيانات النشر: | Springer Science and Business Media LLC, 1989. |
| سنة النشر: | 1989 |
| مصطلحات موضوعية: | food.ingredient, Polyacrylamide, Formaldehyde, Analytical chemistry, Gelatin, Fixatives, Mice, chemistry.chemical_compound, food, Phenols, Animals, Humans, Phenol, Polyacrylamide gel electrophoresis, Fixative, Fixation (histology), Gel electrophoresis, Chromatography, Histocytochemistry, Chemistry, Histological Techniques, Cell Biology, Microscopy, Electron, Anatomy |
| الوصف: | The addition of 2% phenol had a marked accelerating effect on neutral buffered 4% formaldehyde as a fixative. Histopathological material fixed in buffered phenol-formaldehyde (pH 7.0) and rapidly advanced to paraffin in an enclosed tissue-processor showed improved nuclear and cytoplasmic detail, reduced shrinkage and distortion, and an absence of formalin pigment. Good results were obtained in less time when sequential fixation in phenol-formaldehyde buffered to pH 7.0 and pH 5.5 was carried out at an elevated temperature (40 degrees C) in the enclosed tissue-processor. Standard histological stains and immunoperoxidase methods worked well. In resin-embedded tissue, buffered phenol-formaldehyde (pH 7.0) gave satisfactory ultrastructural results. The penetration rate of buffered phenol-formaldehyde (pH 7.0) in gelatin models did not differ from that of neutral buffered 4% formaldehyde. Polyacrylamide gel electrophoresis showed enhanced protein polymer formation with buffered phenol-formaldehyde (pH 7.0) as compared with neutral buffered 4% formaldehyde. Protein polymer formation increased in response to increased time and temperature. Cells fixed in suspension in buffered phenol-formaldehyde (pH 7.0) and neutral buffered 4% formaldehyde showed similar volume changes. |
| تدمد: | 1573-6865 0018-2214 |
| DOI: | 10.1007/bf01747525 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0e8dd234ef044eec357a7453493b2f06 https://doi.org/10.1007/bf01747525 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....0e8dd234ef044eec357a7453493b2f06 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15736865 00182214 |
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| DOI: | 10.1007/bf01747525 |