Characterization of the proteome and metabolome of human liver sinusoidal endothelial-like cells derived from induced pluripotent stem cells
| العنوان: | Characterization of the proteome and metabolome of human liver sinusoidal endothelial-like cells derived from induced pluripotent stem cells |
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| المؤلفون: | Benedikt Scheidecker, Marjorie Leduc, Françoise Gilard, Rachid Jellali, Eric Leclerc, Bertrand Gakière, Atsushi Miyajima, Mathieu Danoy, Taketomo Kido, Yasuyuki Sakai, Fabrice Soncin, Johanna Bruce, Yannick Tauran |
| المساهمون: | Laboratory for Integrated Micro Mechatronics Systems (LIMMS), The University of Tokyo (UTokyo)-Centre National de la Recherche Scientifique (CNRS), The University of Tokyo (UTokyo), Biomécanique et Bioingénierie (BMBI), Université de Technologie de Compiègne (UTC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire des Multimatériaux et Interfaces (LMI), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Plateforme protéomique 3P5 [Institut Cochin] (3P5), Institut Cochin (IC UM3 (UMR 8104 / U1016)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP), Institut des Sciences des Plantes de Paris-Saclay (IPS2 (UMR_9213 / UMR_1403)), Université d'Évry-Val-d'Essonne (UEVE)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Centre Régional de Lutte contre le Cancer Oscar Lambret [Lille] (UNICANCER/Lille), Université Lille Nord de France (COMUE)-UNICANCER, Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Université d'Évry-Val-d'Essonne (UEVE)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université de Lille-UNICANCER, LIMMS internal fundingFEDER through the « Operational Programme for Competitiveness Factors and employment 2007-2013 », ANR-16-RHUS-0005,iLite,iLite(2016), JELLALI, RACHID, iLite - - iLite2016 - ANR-16-RHUS-0005 - RHUS - VALID |
| المصدر: | Differentiation Differentiation, Elsevier, 2021, 120, pp.28-35. ⟨10.1016/j.diff.2021.06.001⟩ Differentiation, 2021, 120, pp.1-26. ⟨10.1016/j.diff.2021.06.001⟩ |
| بيانات النشر: | Elsevier BV, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0301 basic medicine, Cancer Research, Proteome, Angiogenesis, Induced Pluripotent Stem Cells, LSECs, Biology, 03 medical and health sciences, 0302 clinical medicine, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], Metabolome, Humans, Liver Sinusoidal Endothelial Cells, Induced pluripotent stem cell, Molecular Biology, Cells, Cultured, proteomic, hiPSCs, CD40, Wnt signaling pathway, Endothelial Cells, Cell Differentiation, [SDV.MHEP.HEG]Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology, Cell Biology, [SDV.MHEP.HEG] Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology, Cell biology, human induced pluripotent stem cells, Endothelial stem cell, 030104 developmental biology, Liver, biology.protein, Hepatic stellate cell, [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], Endothelium, Vascular, 030217 neurology & neurosurgery, Mannose receptor, metabolomic, Developmental Biology |
| الوصف: | International audience; The liver is a complex organ composed of several cell types organized hierarchically. Among these, liver sinusoidal endothelial cells (LSECs) are specialized vascular cells known to interact with hepatocytes and hepatic stellate cells (HSCs), and to be involved in the regulation of important hepatic processes in healthy and pathological situations. Protocols for the differentiation of LSECs from human induced pluripotent stem cells, hiPSCs, have been proposed and in-depth analysis by transcriptomic profiling of those cells has been performed. In the present work, an extended analysis of those cells in terms of proteome and metabolome has been implemented. The proteomic analysis confirmed the expression of important endothelial markers and pathways. Among them, the expression of patterns typical of LSECs such as PECAM1, VWF, LYVE1, STAB1 (endothelial markers), CDH13, CDH5, CLDN5, ICAM1, MCAM-CD146, ICAM2, ESAM (endothelial cytoskeleton), NOSTRIN, NOS3 (Nitric Oxide endothelial ROS), ESM1, ENG, MMRN2, THBS1, ANGPT2 (angiogenesis), CD93, MRC1 (mannose receptor), CLEC14A (C-type lectin), CD40 (antigen), and ERG (transcription factor) was highlighted. Besides, the pathway analysis revealed the enrichment of the endocytosis, Toll-like receptor, Nod-like receptor, Wnt, Apelin, VEGF, cGMP-PCK, and PPAR related signaling pathways. Other important pathways such as vasopressin regulated water reabsorption, fluid shear stress, relaxin signaling, and renin secretion were also highlighted. At confluence, the metabolome profile appeared consistent with quiescent endothelial cell patterns. The integration of both proteome and metabolome datasets revealed a switch from fatty acid synthesis in undifferentiated hiPSCs to a fatty oxidation in LSECs and activation of the pentose phosphate pathway and polyamine metabolism in hiPSCs-derived LSECs. In conclusion, the comparison between the signature of LSECs differentiated following the protocol described in this work, and data found in the literature confirmed the particular relevance of these cells for future in vitro applications. |
| وصف الملف: | application/pdf |
| تدمد: | 0301-4681 1432-0436 |
| DOI: | 10.1016/j.diff.2021.06.001 |
| DOI: | 10.1016/j.diff.2021.06.001⟩ |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0e74998e41866c9c53fae07a57defad0 https://doi.org/10.1016/j.diff.2021.06.001 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....0e74998e41866c9c53fae07a57defad0 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 03014681 14320436 |
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| DOI: | 10.1016/j.diff.2021.06.001 |