Assessment of airway distribution of transnasal solutions in mice by PET/CT imaging
| العنوان: | Assessment of airway distribution of transnasal solutions in mice by PET/CT imaging |
|---|---|
| المؤلفون: | Maria L. Baeza, Manuel Desco, L. Conejero, María Luisa Soto-Montenegro, J.J. Vaquero, José Manuel Zubeldia |
| المصدر: | e-Archivo. Repositorio Institucional de la Universidad Carlos III de Madrid instname |
| بيانات النشر: | Springer, 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | Cancer Research, medicine.medical_specialty, Quantitative imaging, FDG, Pet ct imaging, Statistics, Nonparametric, Allergen challenge, Mice, Fluorodeoxyglucose F18, medicine, Animals, Distribution (pharmacology), Tissue Distribution, Whole Body Imaging, Radiology, Nuclear Medicine and imaging, Lung, Bronchial asthma, Administration, Intranasal, Biología y Biomedicina, Transnasal administration, Analysis of Variance, Mice, Inbred BALB C, medicine.diagnostic_test, business.industry, Significant difference, Asthma, medicine.anatomical_structure, PET, Oncology, Positron emission tomography, Positron-Emission Tomography, Female, Radiology, Tomography, X-Ray Computed, Airway, business, CT |
| الوصف: | Transnasal administration is one of the most common routes for allergen challenge in mouse models of airway diseases. Although this technique is widely used, neither the amount of allergen that reaches the lung nor its airway distribution has been well established. We used positron emission tomography (PET) and computed tomography (CT) to examine the anatomical distribution of a solution containing a tracer immediately after transnasal delivery and to determine the possible influence of age and administered volume. Procedures: Forty-six female BALB/c mice were divided into three groups according to instillation volume and age: (A) 15 μl, 8–10 weeks old (N=10), (B) 30 μl, 8–10 weeks old (N=20), and (C) 30 μl, 32 weeks old (N=16). Anesthetized animals underwent a dynamic scan in a dedicated small-animal PET scanner immediately after transnasal administration of a solution containing 18FDG. Regions of interest were used to obtain quantitative data. Animals were also imaged with a small-animal CT scanner to obtain complementary anatomical information. Results: Mean±SD (5.69±4.51%) of the solution administered reached the lungs in group A,41.84±8.03% in group B, and 36.65±16.15% in group C. A comparable percentage was delivered to the left and right lungs in all the groups. Analysis of variance revealed a significant difference between the groups in the proportion of the solution that reached the lungs depending on the injection volume (PG0.001), but not depending on animal age. Conclusions: In this first report on quantitative imaging by PET and CT in small animals, we confirmed the suitability of the transnasal route with an instilled volume of 30 μl delivering fluids into the lower airways, although only about 40% of the dose reaches the lungs This work was supported by grants from Ministerio de Ciencia y Tecnología (TEC2004-07052-C02-01), Ministerio de Sanidad y Consumo (CIBER CB06/01/0079 CIBER CB07/09/0031 and FIS 2001/0598), Ministerio de Industria (CDTEAM Project) and Fundación de Investigación Médica Mutua Madrileña Publicado |
| وصف الملف: | application/pdf |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0e0e1c1a7757d4dc13418f6a67cdc79b https://hdl.handle.net/10016/12022 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....0e0e1c1a7757d4dc13418f6a67cdc79b |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |