Design of Stable and Uniform Single Nanoparticle Photonics for In Vivo Dynamics Imaging of Nanoenvironments of Zebrafish Embryonic Fluids
| العنوان: | Design of Stable and Uniform Single Nanoparticle Photonics for In Vivo Dynamics Imaging of Nanoenvironments of Zebrafish Embryonic Fluids |
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| المؤلفون: | Xiao-Hong Nancy Xu, Kerry J. Lee, Prakash D. Nallathamby |
| المصدر: | ACS Nano. 2:1371-1380 |
| بيانات النشر: | American Chemical Society (ACS), 2008. |
| سنة النشر: | 2008 |
| مصطلحات موضوعية: | Materials science, Dispersity, General Engineering, Nanophotonics, Contrast Media, General Physics and Astronomy, Nanoparticle, Nanotechnology, Chorion, Image Enhancement, Article, Silver nanoparticle, Body Fluids, Viscosity, Ionic strength, Biophysics, Animals, Nanoparticles, Particle, General Materials Science, Surface plasmon resonance, Zebrafish |
| الوصف: | We report here, for the first time, the use of a simple washing approach to reduce the ionic strength of the solution, which increased thickness of electric double layer on the surface of silver (Ag) nanoparticles, and thereby enhanced their surface zeta potential. This approach allowed us to prepare optically uniform (75–99%) and purified Ag nanoparticles (11.3 ± 2.3 nm) that are stable (non aggregation) in solution for months, permitting them to become robust and widely-used single nanoprobes for in vivo optical imaging. These Ag nanoparticles show remarkable photostability and serve as single nanoparticle photonic probes for continuous imaging nano-environments of segmentation-stage zebrafish embryos for hours. Unlike other particle tracking experiments, we utilized size-dependent localized-surface-plasmon-resonance-spectra (LSPRS) (colors) of single Ag nanoparticles to determine given colored (sized) nanoparticles in situ and used the monodisperse color (size) of nanoparticles to simultaneously measure viscosities and flow patterns of multiple proximal nano-environments in segmentation-stage zebrafish embryos in real-time. We found new interesting counterclockwise flow patterns with rates ranging from 0.06 to 1.8 μm/s and stunningly high viscosity gradients spanning two-orders of magnitude in chorion space of the embryos, with the highest viscosity observed around the center of chorion space and the lower viscosity at the interfacial areas near the surface of both chorion layers and inner mass of the embryos. This study demonstrates the possibility of using individual monodisperse nano-photonics to probe the roles of embryonic fluid dynamics in embryonic development. |
| تدمد: | 1936-086X 1936-0851 |
| DOI: | 10.1021/nn800048x |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0dd18d70c2ec8a423fde6d7c67042a9e https://doi.org/10.1021/nn800048x |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....0dd18d70c2ec8a423fde6d7c67042a9e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1936086X 19360851 |
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| DOI: | 10.1021/nn800048x |