Antigen-presenting human B cells are expanded in inflammatory conditions
| العنوان: | Antigen-presenting human B cells are expanded in inflammatory conditions |
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| المؤلفون: | Hinrich Abken, Dominik Wolf, Michael von Bergwelt-Baildon, Andrea Rubbert-Roth, Kerstin Wennhold, Rieke Fischer, Gunter Rappl, Maria Garcia-Marquez, Clara Lehmann, Michael Hallek, Marco Herling, Sebastian Theurich, Anne Fiedler, Juliane Iltgen-Breburda, Alexander Shimabukuro-Vornhagen, Gerd Fätkenheuer |
| المصدر: | Journal of Leukocyte Biology. 101:577-587 |
| بيانات النشر: | Oxford University Press (OUP), 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Adult, 0301 basic medicine, CD40 Ligand, Immunology, B-Lymphocyte Subsets, Antigen-Presenting Cells, Down-Regulation, Receptors, Antigen, B-Cell, Inflammation, Lymphocyte Activation, Immunophenotyping, 03 medical and health sciences, Antigen, Cell surface receptor, medicine, Humans, Immunology and Allergy, B cell, Cell Proliferation, CD86, Antigen Presentation, B-Lymphocytes, biology, Vaccination, Cell Biology, medicine.disease, 030104 developmental biology, medicine.anatomical_structure, Rheumatoid arthritis, biology.protein, Receptors, Chemokine, Receptors, Complement 3d, B7-2 Antigen, medicine.symptom, Antibody, Function (biology), Signal Transduction |
| الوصف: | Traditionally, B cells have been best known for their role as producers of antibodies. However, in recent years, a growing body of evidence has accumulated showing that B cells fulfill a range of other immunologic functions. One of the functions that has attracted increasing attention is the capacity of B cells to induce antigen-specific activation of T cells through presentation of antigens. However, the analysis of this B cell function has been hampered by the lack of a phenotypically well-defined antigen-presenting B cell subset. Here, we report the identification of a human antigen-presenting B cell subset with strong immunostimulatory properties. This B cell subset is characterized by low expression of CD21 and high expression of the activation marker CD86 and exhibits strong T cell–stimulatory activity, as demonstrated by means of an autologous mixed-lymphocyte reaction. Phenotypically, CD21lowCD86pos immunostimulatory B cells (BAPC) represented CD27+ class-switched IgMnegIgDneg B lymphocytes and displayed a higher expression of cell surface receptors, which mediate the migration from peripheral blood to sites of inflammation. Flow cytometric analysis of peripheral blood obtained from individuals with inflammatory conditions revealed that the BAPC subset was expanded following vaccination and in patients with rheumatoid arthritis. Taken together, our work shows that BAPC represents a strongly immunostimulatory B cell subset, which could be a promising target for immunotherapeutic intervention in inflammatory diseases. |
| تدمد: | 1938-3673 0741-5400 |
| DOI: | 10.1189/jlb.5a0416-182r |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0dca7e136a935392a6424bbc3951b7f2 https://doi.org/10.1189/jlb.5a0416-182r |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....0dca7e136a935392a6424bbc3951b7f2 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19383673 07415400 |
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| DOI: | 10.1189/jlb.5a0416-182r |