Otoliths-composed gelatin/sodium alginate scaffolds for bone regeneration
| العنوان: | Otoliths-composed gelatin/sodium alginate scaffolds for bone regeneration |
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| المؤلفون: | Daisy Pereira Valido, Eliana B. Souto, Luiza Abrahão Frank, Adriano Antunes de Souza Araújo, Renata de Lima, Felipe Mendes de Andrade de Carvalho, Eloísa Portugal Barros Silva Soares de Souza, André Polloni, Ana Maria Santos Oliveira, Gabriela das Graças Gomes Trindade, Maria Eliane de Andrade, Ricardo Luiz Cavalcanti de Albuquerque Júnior, Francine Ferreira Padilha, Patrícia Severino, Silvia Stanisçuaski Guterres, Charlene R. S. Matos, Caio de Alcântara Campos, Wilson Déda Gonçalves Júnior, Eliana Midori Sussuchi, A. A. Rezende |
| المساهمون: | Universidade do Minho |
| المصدر: | Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP |
| بيانات النشر: | Springer Science and Business Media LLC, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Thermogravimetric analysis, Bone Regeneration, food.ingredient, Alginates, Scanning electron microscope, Fluorescence spectrometry, Pharmaceutical Science, Biocompatible Materials, 02 engineering and technology, 030226 pharmacology & pharmacy, Gelatin, Mice, Otolithic Membrane, 03 medical and health sciences, 0302 clinical medicine, Differential scanning calorimetry, food, Spectroscopy, Fourier Transform Infrared, medicine, Animals, Tissue engineering, Fourier transform infrared spectroscopy, Bone regeneration, Tissue scaffolds, Science & Technology, Tissue Engineering, Tissue Scaffolds, Chemistry, 3T3 Cells, 021001 nanoscience & nanotechnology, Otoliths, sense organs, Swelling, medicine.symptom, 0210 nano-technology, Porosity, Nuclear chemistry |
| الوصف: | Evidence that otoliths, mineral-rich limestone concrescences present in the inner ear of bone fishes, can accelerate bone formation in vivo has been previously reported. The goal of this work was the development, characterization, and evaluation of the cytocompatibility of otoliths-incorporated sodium alginate and gelatin scaffolds. Cynoscion acoupaderived otoliths were characterized by X-ray fluorescence spectrometry (FRX), particle size, free lime, and weight loss by calcination. Furthermore, otoliths were incorporated into sodium alginate (ALG/OTL-s) or gelatin (GEL/OTL-s) scaffolds, previously developed by freeze-drying. Then, the scaffolds were characterized by thermogravimetric analysis (TGA/DTG), differential scanning calorimetry (DSC), infrared spectroscopy with Fourier transform (FTIR), swelling tests, and scanning electron microscopy (SEM). Cytotoxicity assays were run against J774.G8 macrophages and MC3T3-E1 osteoblasts. Data obtained from TGA/DTG, DSC, and FTIR analyses confirmed the interaction between otoliths and the polymeric scaffolds. SEM showed the homogeneous porous 3D structure rich in otolith micro-fragments in both scaffolds. Swelling of the GEL/OTL-s (63.54±3.0%) was greater than of ALG/OTL-s (13.36±9.9%) (p0.05) and significantly higher than that treated with Triton-X (p0.05). However, by 48 h, only ALG/OTL-s showed growth similar to control (p>0.05), whereas GEL/OTL showed a significantly lower growth index (p We would like to thank the National Council for Scientific and Technological Development (CNPq) and the Foundation for Research and Technological Innovation Support of the State of Sergipe for the financial support in this study. EMBS acknowledges the sponsorship of the projects M-ERA-NET-0004/2015-PAIRED and UIDB/04469/2020 (strategic fund), received support from the Portuguese Science and Technology Foundation, Ministry of Science and Education (FCT/MEC) through national funds, and was co-financed by FEDER, under the Partnership Agreement PT2020. info:eu-repo/semantics/publishedVersion |
| وصف الملف: | application/pdf |
| تدمد: | 2190-3948 2190-393X |
| DOI: | 10.1007/s13346-020-00845-x |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0d0e2f7fd1e797d33644792c70844186 https://doi.org/10.1007/s13346-020-00845-x |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....0d0e2f7fd1e797d33644792c70844186 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 21903948 2190393X |
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| DOI: | 10.1007/s13346-020-00845-x |