Increasing the relative expression of endogenous non-coding Steroid Receptor RNA Activator (SRA) in human breast cancer cells using modified oligonucleotides
| العنوان: | Increasing the relative expression of endogenous non-coding Steroid Receptor RNA Activator (SRA) in human breast cancer cells using modified oligonucleotides |
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| المؤلفون: | Charlton Cooper, Jimin Guo, Yvonne Myal, Yi Yan, Etienne Leygue, Florent Hubé, Mohammad K. Hamedani, Leigh C. Murphy, Shilpa Chooniedass-Kothari |
| المساهمون: | Université Paris Diderot - Paris 7 (UPD7) |
| المصدر: | Nucleic Acids Research Nucleic Acids Research, Oxford University Press, 2009, 37 (13), pp.4518-4531. ⟨10.1093/nar/gkp441⟩ |
| بيانات النشر: | HAL CCSD, 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | RNA, Untranslated, [SDV]Life Sciences [q-bio], Estrogen receptor, Breast Neoplasms, Biology, 03 medical and health sciences, 0302 clinical medicine, Cell Line, Tumor, Progesterone receptor, Genetics, Humans, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Molecular Biology, Transcription factor, Estrogen receptor beta, ComputingMilieux_MISCELLANEOUS, Cell Proliferation, 030304 developmental biology, Regulation of gene expression, 0303 health sciences, Estradiol, Activator (genetics), Alternative splicing, Intron, Introns, Gene Expression Regulation, Neoplastic, Alternative Splicing, 030220 oncology & carcinogenesis, Cancer research, Female, RNA, Long Noncoding, Receptors, Progesterone, Oligoribonucleotides, Antisense |
| الوصف: | Products of the Steroid Receptor RNA Activator gene (SRA1) have the unusual property to modulate the activity of steroid receptors and other transcription factors both at the RNA (SRA) and the protein (SRAP) level. Balance between these two genetically linked entities is controlled by alternative splicing of intron-1, whose retention alters SRAP reading frame. We have previously found that both fully-spliced SRAP-coding and intron-1-containing non-coding SRA RNAs co-exist in breast cancer cell lines. Herein, we report a significant (Student's t-test, P < 0.003) higher SRA–intron-1 relative expression in breast tumors with higher progesterone receptor contents. Using an antisense oligoribonucleotide, we have successfully reprogrammed endogenous SRA splicing and increased SRA RNA–intron-1 relative level in T5 breast cancer cells. This increase is paralleled by significant changes in the expression of genes such as plasminogen urokinase activator and estrogen receptor beta. Estrogen regulation of other genes, including the anti-metastatic NME1 gene, is also altered. Overall, our results suggest that the balance coding/non-coding SRA transcripts not only characterizes particular tumor phenotypes but might also, through regulating the expression of specific genes, be involved in breast tumorigenesis and tumor progression. |
| اللغة: | English |
| تدمد: | 0305-1048 1362-4962 |
| DOI: | 10.1093/nar/gkp441⟩ |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0cf534fbdf8cef528d71b8e500de41da https://hal-univ-paris.archives-ouvertes.fr/hal-02127334 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....0cf534fbdf8cef528d71b8e500de41da |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 03051048 13624962 |
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| DOI: | 10.1093/nar/gkp441⟩ |