Transcriptional profiles of unirradiated or UV-irradiated human cells expressing either the cancer-prone XPB/CS allele or the noncancer-prone XPB/TTD allele
| العنوان: | Transcriptional profiles of unirradiated or UV-irradiated human cells expressing either the cancer-prone XPB/CS allele or the noncancer-prone XPB/TTD allele |
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| المؤلفون: | Alain Sarasin, Apuã C.M. Paquola, Renata M. A. Costa, Lydia Riou, Carlos Frederico Martins Menck |
| المصدر: | Oncogene. 24:1359-1374 |
| بيانات النشر: | Springer Science and Business Media LLC, 2004. |
| سنة النشر: | 2004 |
| مصطلحات موضوعية: | Cancer Research, Xeroderma pigmentosum, DNA Repair, Transcription, Genetic, Ultraviolet Rays, DNA repair, Trichothiodystrophy, Down-Regulation, Biology, Transfection, medicine.disease_cause, Cell Line, Neoplasms, Genetics, medicine, Humans, Allele, Cockayne Syndrome, Molecular Biology, Alleles, Oligonucleotide Array Sequence Analysis, Xeroderma Pigmentosum, Gene Expression Profiling, DNA Helicases, Syndrome, Fibroblasts, Cell cycle, medicine.disease, Isogenic human disease models, Molecular biology, Up-Regulation, DNA-Binding Proteins, Mutation, Hair Diseases, Carcinogenesis, Nucleotide excision repair |
| الوصف: | Xeroderma pigmentosum (XP) and trichothiodystrophy (TTD) syndromes are characterized by deficiency in nucleotide excision repair pathway, but with distinguished clinical manifestations. While XP patients exhibit a high frequency of skin cancer, TTD patients are not cancer prone. The relation between lack of DNA repair and their clinical manifestations was investigated through analysis of the transcriptional profile of 12,600 transcripts in two isogenic cell lines with different capabilities of DNA repair. These cell lines result from a stable transfection of the XPB-TTD allele into XP complementation group B fibroblasts, from an XP patient who also have clinical abnormalities corresponding to Cockayne's syndrome (CS). The microarray assays performed under normal growth conditions showed the expression of distinct groups of genes in each cell line. The UVC-transcription modulation of these cells revealed the changes in 869 transcripts. Some of these transcripts had similar modulation pattern in both cells, although with eventually different time patterns for induction or repression. However, some different 'UVC signature' for each cell line was also found, that is, transcripts that were specifically UV regulated depending on the DNA repair status of the cell. These results provide a detailed portrait of expression profiles that may potentially unravel the causes of the different phenotypes of XP/CS and TTD patients. |
| تدمد: | 1476-5594 0950-9232 |
| DOI: | 10.1038/sj.onc.1208288 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0c58cbe92010a6d4bbcb6d6de777598f https://doi.org/10.1038/sj.onc.1208288 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....0c58cbe92010a6d4bbcb6d6de777598f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14765594 09509232 |
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| DOI: | 10.1038/sj.onc.1208288 |