American Association for Cancer Research (AACR), 2023.
سنة النشر:
2023
الوصف:
Supplementary Materials and Methods: Immunohistochemistry, Immunofluorescence, TUNEL staining Supplementary Figure S1. Treatment with MI-773 and/or cisplatin is not toxic to mice. Graph depicting mouse weight during the experimental period. Data were normalized against pre-treatment weight. Supplementary Figure S2. Representative photomicrographs of UM-HACC-5 and -6 cells exposed to 1 μM MI-773 and/or 2 μM cisplatin, or vehicle control, for 72 hours in vitro. Supplementary Figure S3. Representative graphs depicting the number of UM-HACC-5 (A) and -6 (B) cells in each cell-cycle phase after treatment with 1 μM MI-773 and/or 2 μM cisplatin, or vehicle control, for 24 hours. Supplementary Figure S4. Representative photomicrographs obtained by immunofluorescence for ALDH (green) in UM-PDX-HACC-5 tumors treated with MI-773 and/or cisplatin. ALDH-positive cells are predominantly observed in vehicle and cisplatin groups (details) and are apparently located in close proximity to blood vessels (*) and nerve structures (N). Scale bar represents 50 μm (400x).