Polycyclic aromatic hydrocarbon-DNA adducts in lung tissue from lung cancer patients
| العنوان: | Polycyclic aromatic hydrocarbon-DNA adducts in lung tissue from lung cancer patients |
|---|---|
| المؤلفون: | Michel J.X. Hillebrand, E. Kriek, N. van Zandwijk, J.T. Lutgerink, F.J. van Schooten, H.M. Jansen, F.E. van Leeuwen |
| المساهمون: | Other departments |
| المصدر: | ResearcherID Carcinogenesis, 11(9), 1677-1681. Oxford University Press |
| بيانات النشر: | Oxford University Press (OUP), 1990. |
| سنة النشر: | 1990 |
| مصطلحات موضوعية: | Cancer Research, Pathology, medicine.medical_specialty, Lung Neoplasms, 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide, Polycyclic aromatic hydrocarbon, Enzyme-Linked Immunosorbent Assay, Dihydroxydihydrobenzopyrenes, Adduct, DNA Adducts, chemistry.chemical_compound, DNA adduct, medicine, Humans, Polycyclic Compounds, Lung cancer, Lung, Carcinogen, chemistry.chemical_classification, Smoking, DNA, DNA, Neoplasm, General Medicine, Environmental exposure, medicine.disease, Molecular biology, chemistry, Autoradiography, Pyrene, Phosphorus Radioisotopes |
| الوصف: | In an attempt to probe for polycyclic aromatic hydrocarbon (PAH)-DNA adducts in human subjects resulting from smoking (or other chronic environmental exposure), lung tissue and lung tumours were obtained from patients hospitalized for lung cancer. DNA was isolated from the tissue samples and examined both in an ELISA using a polyclonal antibody against (+/-)trans-7,8-dihydroxy-anti-9,10-epoxy-7,8,9,10- tetrahydrobenzo[a]pyrene (BPDE)-DNA as well as by the nuclease P1-mediated modification of the 32P-post-labelling technique. The ELISA results showed BPDE-DNA antigenicity in lung DNA from 6 out of 21 patients, and adduct levels ranged from 2 to 134 adducts per 10(8) nucleotides. For all 21 patients, the autoradiographs of chromatograms of 32P-postlabelled digests of DNA from non-tumorous lung tissue showed a strong diagonal radioactive zone (DRZ). This DRZ was generally absent in tumorous tissue. DNA samples that were positive in the ELISA contained a dominant spot within the DRZ that co-chromatographed with the major BPDE-DNA adduct (BPDE-dG). The quantities of the BPDE-dG spots ranged from 2.1 to 42 adducts in 10(9) nucleotides. These values were lower than the levels found in the ELISA but correlated well with the ELISA results (Kendall W = 0.97; P = 0.00). The levels of the DRZ adducts ranged from 1.9 to 34 adducts in 10(8) nucleotides. Correlations between smoking and DNA adduct levels were poor because of the small number of current smokers (n = 13). However, smokers of filter cigarettes had significantly lower DNA adduct levels compared with smokers of cigarettes without a filter (P = 0.02 by Fischer's exact test). |
| تدمد: | 1460-2180 0143-3334 |
| DOI: | 10.1093/carcin/11.9.1677 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0b15fb386b00014857a2de495590e75e https://doi.org/10.1093/carcin/11.9.1677 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....0b15fb386b00014857a2de495590e75e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14602180 01433334 |
|---|---|
| DOI: | 10.1093/carcin/11.9.1677 |