Large-scale curvature sensing by directional actin flow drives cellular migration mode switching
| العنوان: | Large-scale curvature sensing by directional actin flow drives cellular migration mode switching |
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| المؤلفون: | Agustí Brugués, Benoit Ladoux, Hui Ting Ong, Xavier Trepat, Yusuke Toyama, E. G. Fedorov, Tianchi Chen, Boon Chuan Low, Andrew Callan-Jones, Raphaël Voituriez, Andrea Ravasio, Tom Shemesh |
| المساهمون: | National University of Singapore (NUS), Physico-Chimie-Curie (PCC), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut Curie [Paris]-Centre National de la Recherche Scientifique (CNRS), S.I. Vavilov State Optical Institute, Mechanobiology Institute [Singapore] (MBI), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU), Temasek life sciences laboratory [Singapore], Institució Catalana de Recerca i Estudis Avançats (ICREA), Laboratoire de Physique Théorique de la Matière Condensée (LPTMC), Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC), Matière et Systèmes Complexes (MSC (UMR_7057)), Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Technion - Israel Institute of Technology [Haifa], Institut de Bioenginyeria de Catalunya, ICREA (IBEC), Universitat de Barcelona (UB)-Facultat de Medicina, Barcelona Institute of Science and Technology (BIST), Laboratoire Jean Perrin (LJP), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Paris Seine (IBPS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut Jacques Monod (IJM (UMR_7592)), Centre National de la Recherche Scientifique (CNRS)-Université Paris Diderot - Paris 7 (UPD7), Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) |
| المصدر: | Nature physics Nature Physics Nature Physics, Nature Publishing Group, 2019, 15 (4), pp.393-402. ⟨10.1038/s41567-018-0383-6⟩ |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | cell migration, [PHYS.PHYS.PHYS-BIO-PH]Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph], [SDV]Life Sciences [q-bio], General Physics and Astronomy, curvature sensing, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, macromolecular substances, Curvature, 01 natural sciences, Article, 010305 fluids & plasmas, Focal adhesion, Mechanobiology, 0103 physical sciences, 010306 general physics, Actin, ComputingMilieux_MISCELLANEOUS, [PHYS]Physics [physics], Physics, actin flow, Cell migration, biophysical modelling, mechanobiology, Actin cytoskeleton, Biophysics, Mode switching, Wound healing |
| الوصف: | International audience; Cell migration over heterogeneous substrates during wound healing or morphogenetic processes leads to shape changes driven by different organizations of the actin cytoskeleton and by functional changes including lamellipodial protrusions and contractile actin cables. Cells distinguish between cell-sized positive and negative curvatures in their physical environment by forming protrusions at positive ones and actin cables at negative ones; however, the cellular mechanisms remain unclear. Here, we report that concave edges promote polarized actin structures with actin flow directed towards the cell edge, in contrast to well-documented retrograde flow at convex edges. Anterograde flow and contractility induce a tension anisotropy gradient. A polarized actin network is formed, accompanied by a local polymerization-depolymerization gradient, together with leading-edge contractile actin cables in the front. These cables extend onto non-adherent regions while still maintaining contact with the substrate through focal adhesions. The contraction and dynamic reorganization of this actin structure allows forward movements enabling cell migration over non-adherent regions on the substrate. These versatile functional structures may help cells sense and navigate their environment by adapting to external geometric and mechanical cues. |
| تدمد: | 1745-2473 1476-4636 |
| DOI: | 10.1038/s41567-018-0383-6⟩ |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0911f669c54e657281ddfbab76ffbb88 https://pubmed.ncbi.nlm.nih.gov/30984281 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....0911f669c54e657281ddfbab76ffbb88 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17452473 14764636 |
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| DOI: | 10.1038/s41567-018-0383-6⟩ |