Multiscale imaging of basal cell dynamics in the functionally mature mammary gland
| العنوان: | Multiscale imaging of basal cell dynamics in the functionally mature mammary gland |
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| المؤلفون: | James W. Putney, Alexander J. Stevenson, Gilles Vanwalleghem, Teneale A. Stewart, Ethan K. Scott, Adam D. Ewing, Bethan Lloyd-Lewis, Natascia Marino, Nicholas D. Condon, Felicity M. Davis |
| المصدر: | Stevenson, A J, Vanwalleghem, G, Stewart, T A, Condon, N D, Lloyd-Lewis, B, Marino, N, Putney, J W, Scott, E K, Ewing, A D & Davis, F M 2020, ' Multiscale imaging of basal cell dynamics in the functionally mature mammary gland ', Proceedings of the National Academy of Sciences of the United States of America, vol. 117, no. 43, pp. 26822-26832 . https://doi.org/10.1073/pnas.2016905117 Proc Natl Acad Sci U S A Stevenson, A J, Vanwalleghem, G, Stewart, T A, Condon, N D, Lloyd-Lewis, B, Marino, N, Putney, J W, Scott, E K, Ewing, A D & Davis, F M 2020, ' Multiscale imaging of basal cell dynamics in the functionally mature mammary gland ', Proceedings of the National Academy of Sciences, vol. 117, no. 43, pp. 26822-26832 . https://doi.org/10.1073/pnas.2016905117 |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Genetically modified mouse, mammary gland, Myosin Light Chains, Intravital Microscopy, Mammary gland, Blood volume, Mice, Transgenic, lactation, Stimulus (physiology), GCaMP6, calcium signaling, Mice, Mammary Glands, Animal, Lactation, oxytocin, medicine, Animals, Humans, Calcium Signaling, Milk Ejection, Mammary Glands, Human, Calcium signaling, Multidisciplinary, Chemistry, Epithelial Cells, Biological Sciences, Cell biology, medicine.anatomical_structure, Mammary Epithelium, Oxytocin, medicine.drug |
| الوصف: | The mammary epithelium is indispensable for the continued survival of more than 5,000 mammalian species. For some, the volume of milk ejected in a single day exceeds their entire blood volume. Here, we unveil the spatiotemporal properties of physiological signals that orchestrate the ejection of milk from alveolar units and its passage along the mammary ductal network. Using quantitative, multidimensional imaging of mammary cell ensembles from GCaMP6 transgenic mice, we reveal how stimulus evoked Ca(2+) oscillations couple to contractions in basal epithelial cells. Moreover, we show that Ca(2+)-dependent contractions generate the requisite force to physically deform the innermost layer of luminal cells, compelling them to discharge the fluid that they produced and housed. Through the collective action of thousands of these biological positive-displacement pumps, each linked to a contractile ductal network, milk begins its passage toward the dependent neonate, seconds after the command. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| DOI: | 10.1073/pnas.2016905117 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::07e677f1abc68484b926a7f5a20053ca https://research-information.bris.ac.uk/ws/files/254280155/PNAS_no2019_14952_supp_20200817_compiled.pdf |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....07e677f1abc68484b926a7f5a20053ca |
| قاعدة البيانات: | OpenAIRE |
| DOI: | 10.1073/pnas.2016905117 |
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