The complex resistomes of Paenibacillaceae reflect diverse antibiotic chemical ecologies
| العنوان: | The complex resistomes of Paenibacillaceae reflect diverse antibiotic chemical ecologies |
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| المؤلفون: | Gerard D. Wright, Andrew C. Pawlowski, Erin L. Westman, Nicholas Waglechner, Kalinka Koteva |
| المصدر: | The ISME Journal. 12:885-897 |
| بيانات النشر: | Springer Science and Business Media LLC, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, 030106 microbiology, Biology, Microbiology, Article, 03 medical and health sciences, Antibiotic resistance, Phylogenomics, Drug Resistance, Bacterial, Humans, Gene, Ecology, Evolution, Behavior and Systematics, Brevibacillus, Ecology, Gene Expression Profiling, Paenibacillaceae, biology.organism_classification, Anti-Bacterial Agents, Resistome, Caves, 030104 developmental biology, Evolutionary biology, Mobile genetic elements, Paenibacillus, Bacteria |
| الوصف: | The ecology of antibiotic resistance involves the interplay of a long natural history of antibiotic production in the environment, and the modern selection of resistance in pathogens through human use of these drugs. Important components of the resistome are intrinsic resistance genes of environmental bacteria, evolved and acquired over millennia, and their mobilization, which drives dissemination in pathogens. Understanding the dynamics and evolution of resistance across bacterial taxa is essential to address the current crisis in drug-resistant infections. Here we report the exploration of antibiotic resistance in the Paenibacillaceae prompted by our discovery of an ancient intrinsic resistome in Paenibacillus sp. LC231, recovered from the isolated Lechuguilla cave environment. Using biochemical and gene expression analysis, we have mined the resistome of the second member of the Paenibacillaceae family, Brevibacillus brevis VM4, which produces several antimicrobial secondary metabolites. Using phylogenomics, we show that Paenibacillaceae resistomes are in flux, evolve mostly independent of secondary metabolite biosynthetic diversity, and are characterized by cryptic, redundant, pseudoparalogous, and orthologous genes. We find that in contrast to pathogens, mobile genetic elements are not significantly responsible for resistome remodeling. This offers divergent modes of resistome development in pathogens and environmental bacteria. |
| تدمد: | 1751-7370 1751-7362 |
| DOI: | 10.1038/s41396-017-0017-5 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::075f5a236b70d1231c8e32b32f834c77 https://doi.org/10.1038/s41396-017-0017-5 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....075f5a236b70d1231c8e32b32f834c77 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17517370 17517362 |
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| DOI: | 10.1038/s41396-017-0017-5 |