Cryofracture electron microscopy of the ookinete pellicle of Plasmodium gallinaceum reveals the existence of novel pores in the alveolar membranes
| العنوان: | Cryofracture electron microscopy of the ookinete pellicle of Plasmodium gallinaceum reveals the existence of novel pores in the alveolar membranes |
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| المؤلفون: | Paul T. Brey, Richard Paul, John E. Heuser, Robert E. Sinden, Anna Raibaud, Romano Dallai, David Mercati, Pietro Lupetti |
| المساهمون: | Biochimie et Biologie Moléculaire des Insectes, Institut Pasteur [Paris] (IP), Università degli Studi di Siena = University of Siena (UNISI), Imperial College London, Washington University School of Medicine in St. Louis, Washington University in Saint Louis (WUSTL) |
| المصدر: | Journal of Structural Biology Journal of Structural Biology, 2001, 135 (1), pp.47-57. ⟨10.1006/jsbi.2001.4396⟩ |
| سنة النشر: | 2001 |
| مصطلحات موضوعية: | plasmodium ultrastructure, Vacuole, Microtubules, Plasmodium gallinaceum, Cell membrane, Structural Biology, Aedes, MESH: Animals, [SDV.MHEP.ME]Life Sciences [q-bio]/Human health and pathology/Emerging diseases, biology, MESH: Microtubules, MESH: Chickens, MESH: Aedes, Cell biology, membrane cytoskeleton, MESH: Intracellular Membranes, Membrane, medicine.anatomical_structure, apicomplexa, MESH: Models, Structural, MESH: Freeze Fracturing, MESH: Imaging, Three-Dimensional, MESH: Microscopy, Electron, quick-freeze deep etching, cell motility, freeze-fracture, MESH: Host-Parasite Interactions, MESH: Vacuoles, Host-Parasite Interactions, Apicomplexa, Imaging, Three-Dimensional, Microtubule, parasitic diseases, medicine, Inner membrane, Animals, Freeze Fracturing, Rhoptry, MESH: Plasmodium gallinaceum, Cell Membrane, deep-etching, Intracellular Membranes, biology.organism_classification, Models, Structural, Microscopy, Electron, quick-freeze, Vacuoles, Chickens, MESH: Cell Membrane |
| الوصف: | International audience; The malaria parasite invades the midgut tissue of its mosquito host as a motile form called the ookinete. We have examined the pellicle of the ookinete of Plasmodium gallinaceum by freeze-fracture and quick-freeze, deep-etch electron microscopy. The general organization is analogous to that of invasive stages of other members of Apicomplexa. The pellicle is composed of three membranes: the plasma membrane, and the two linked intermediate and inner membranes, which in the ookinete form one flattened vacuole that is located beneath the plasma membrane. The edges of this vacuole form a longitudinal suture. Beneath the vacuole is found an array of microtubules that are connected to the inner membrane by intramembranous particles. During freeze-fracture, the membranes can split along their hydrophobic planes, thus yielding six fracture faces, each of which displays a characteristic pattern of intramembranous particles. Additionally, we find that the ookinete pellicle differs from all other apicomplexan motile stages by the presence of large pores. These pores are of unknown function, but clearly might constitute a novel pathway for the transport of molecules to and from the cortex, which is independent of the well-described route through the apical micronemal/rhoptry complex. The pores may be the route by which motor proteins or other non micronemal surface proteins are trafficked, such as P25/P28 and SOAP, some of which are implicated in transmission blocking immunity. |
| تدمد: | 1047-8477 1095-8657 |
| DOI: | 10.1006/jsbi.2001.4396⟩ |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0724fe0d4b95bd49c508f32c446c3ca8 https://pubmed.ncbi.nlm.nih.gov/11562165 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....0724fe0d4b95bd49c508f32c446c3ca8 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10478477 10958657 |
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| DOI: | 10.1006/jsbi.2001.4396⟩ |