A universal transcription pause sequence is an element of initiation factor σ70-dependent pausing
| العنوان: | A universal transcription pause sequence is an element of initiation factor σ70-dependent pausing |
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| المؤلفون: | Jeffrey W. Roberts, Eric J. Strobel, Jeremy G. Bird |
| المصدر: | Nucleic Acids Research |
| بيانات النشر: | Oxford University Press, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, Enzyme complex, Transcription, Genetic, Sigma Factor, Biology, medicine.disease_cause, 03 medical and health sciences, chemistry.chemical_compound, Sigma factor, Transcription (biology), Peptide Initiation Factors, Gene expression, Genetics, medicine, Escherichia coli, Initiation factor, Promoter Regions, Genetic, Base Composition, 030102 biochemistry & molecular biology, Base Sequence, Models, Genetic, Gene regulation, Chromatin and Epigenetics, Nucleic Acid Heteroduplexes, RNA, Templates, Genetic, Bacteriophage lambda, RNA, Bacterial, 030104 developmental biology, chemistry, DNA, Viral, DNA |
| الوصف: | The Escherichia coli σ70 initiation factor is required for a post-initiation, promoter-proximal pause essential for regulation of lambdoid phage late gene expression; potentially, σ70 acts at other sites during transcription elongation as well. The pause is induced by σ70 binding to a repeat of the promoter -10 sequence. After σ70 binding, further RNA synthesis occurs as DNA is drawn (or 'scrunched') into the enzyme complex, presumably exactly as occurs during initial synthesis from the promoter; this synthesis then pauses at a defined site several nucleotides downstream from the active center position when σ70 first engages the -10 sequence repeat. We show that the actual pause site in the stabilized, scrunched complex is the 'elemental pause sequence' recognized from its frequent occurrence in the E. coli genome. σ70 binding and the elemental pause sequence together, but neither alone, produce a substantial transcription pause. |
| اللغة: | English |
| تدمد: | 1362-4962 0305-1048 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::06e1d5ae94449d6efac87a1ebc6e791b http://europepmc.org/articles/PMC5001585 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....06e1d5ae94449d6efac87a1ebc6e791b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13624962 03051048 |
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