A second, non-canonical RNA-dependent RNA polymerase in SARS Coronavirus
| العنوان: | A second, non-canonical RNA-dependent RNA polymerase in SARS Coronavirus |
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| المؤلفون: | Bruno Canard, Bruno Coutard, Isabelle Imbert, François Ferron, Jean Marie Bourhis, Jean-Claude Guillemot, Cécile Bussetta, Alexander E. Gorbalenya, M P Egloff |
| المصدر: | The EMBO Journal |
| بيانات النشر: | Wiley, 2006. |
| سنة النشر: | 2006 |
| مصطلحات موضوعية: | Models, Molecular, viruses, Molecular Sequence Data, RNA-dependent RNA polymerase, Biology, medicine.disease_cause, Genome, Article, General Biochemistry, Genetics and Molecular Biology, Viral Proteins, chemistry.chemical_compound, Transcription (biology), Catalytic Domain, RNA polymerase, medicine, Amino Acid Sequence, Molecular Biology, Polymerase, Coronavirus, Genetics, General Immunology and Microbiology, Oligonucleotide, General Neuroscience, RNA, RNA-Dependent RNA Polymerase, Virology, Protein Structure, Tertiary, Severe acute respiratory syndrome-related coronavirus, chemistry, biology.protein, RNA, Viral |
| الوصف: | In (+) RNA coronaviruses, replication and transcription of the giant approximately 30 kb genome to produce genome- and subgenome-size RNAs of both polarities are mediated by a cognate membrane-bound enzymatic complex. Its RNA-dependent RNA polymerase (RdRp) activity appears to be supplied by non-structural protein 12 (nsp12) that includes an RdRp domain conserved in all RNA viruses. Using SARS coronavirus, we now show that coronaviruses uniquely encode a second RdRp residing in nsp8. This protein strongly prefers the internal 5'-(G/U)CC-3' trinucleotides on RNA templates to initiate the synthesis of complementary oligonucleotides of6 residues in a reaction whose fidelity is relatively low. Distant structural homology between the C-terminal domain of nsp8 and the catalytic palm subdomain of RdRps of RNA viruses suggests a common origin of the two coronavirus RdRps, which however may have evolved different sets of catalytic residues. A parallel between the nsp8 RdRp and cellular DNA-dependent RNA primases is drawn to propose that the nsp8 RdRp produces primers utilized by the primer-dependent nsp12 RdRp. |
| تدمد: | 1460-2075 0261-4189 |
| DOI: | 10.1038/sj.emboj.7601368 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0641bde751077c03e9d1f73141baaea5 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....0641bde751077c03e9d1f73141baaea5 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14602075 02614189 |
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| DOI: | 10.1038/sj.emboj.7601368 |