Isolation of an early T-cell precursor (CFU-TL) from human bone marrow
| العنوان: | Isolation of an early T-cell precursor (CFU-TL) from human bone marrow |
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| المؤلفون: | G. Mouterde, JM Bertho, A H Dalloul, Patrice Debré, M D Mossalayi |
| المصدر: | Blood. 75:1064-1068 |
| بيانات النشر: | American Society of Hematology, 1990. |
| سنة النشر: | 1990 |
| مصطلحات موضوعية: | Antigens, Differentiation, T-Lymphocyte, Time Factors, medicine.drug_class, T-Lymphocytes, T cell, Population, Immunology, CD2 Antigens, Bone Marrow Cells, Antigens, CD7, Cell Separation, Biology, Monoclonal antibody, Cell morphology, Biochemistry, Flow cytometry, Colony-Forming Units Assay, Antigens, CD, medicine, Humans, Receptors, Immunologic, education, education.field_of_study, medicine.diagnostic_test, Cell Differentiation, Cell Biology, Hematology, Flow Cytometry, Hematopoietic Stem Cells, Molecular biology, In vitro, medicine.anatomical_structure, Cell culture, Bone marrow, Cell Division |
| الوصف: | CD2-CD3-CD4-CD8- human bone marrow (BM) cells were previously shown to generate T-cell clones in vitro. This capacity was abolished after treatment of this population with anti-CD7 monoclonal antibody and complement. In this study, using rosetting with sheep erythrocytes, complement-dependent cytotoxicity, and specific immunoadherence method, we isolated a minor BM subset that contained more than 80% CD7+CD2-CD3- CD4-CD8- cells with small lymphoid cell morphology. They comprised most early T-cell precursors (CFU-TL) as they displayed high capacity to generate T-cell clones when cultured in limiting dilutions. CFU-TL nature of these cells was also confirmed by the sequential expression of mature T-cell specific markers on their surface after in vitro induction. This BM subset also contained 2% to 3% CFU-GM precursors. Together, these results pointed to the existence of BM CD7+CD2- precursors with high differentiation potential and showed the commitment of most of them to T-cell lineage. |
| تدمد: | 1528-0020 0006-4971 |
| DOI: | 10.1182/blood.v75.5.1064.bloodjournal7551064 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::05a7976a80da40b391b19eb8fc746339 https://doi.org/10.1182/blood.v75.5.1064.bloodjournal7551064 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....05a7976a80da40b391b19eb8fc746339 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15280020 00064971 |
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| DOI: | 10.1182/blood.v75.5.1064.bloodjournal7551064 |