Antipathogenic and probiotic potential of Lactobacillus brevis strains newly isolated from Algerian artisanal cheeses
| العنوان: | Antipathogenic and probiotic potential of Lactobacillus brevis strains newly isolated from Algerian artisanal cheeses |
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| المؤلفون: | Farah Hosseinian, Aynur Gunenc, Yasmina Ait Chait, Farida Bendali |
| المصدر: | Folia Microbiologica. 66:429-440 |
| بيانات النشر: | Springer Science and Business Media LLC, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Staphylococcus aureus, Salmonella, Levilactobacillus brevis, Biology, medicine.disease_cause, Microbiology, Bacterial Adhesion, law.invention, 03 medical and health sciences, Probiotic, Listeria monocytogenes, Cheese, law, RNA, Ribosomal, 16S, Lactobacillus, Antibiosis, Escherichia coli, medicine, Humans, Colonization, Food science, 030304 developmental biology, 0303 health sciences, 030306 microbiology, Lactobacillus brevis, Probiotics, General Medicine, 16S ribosomal RNA, biology.organism_classification, Food Microbiology, Antagonism, HT29 Cells |
| الوصف: | From 98 Lactobacillus strains, isolated from Algerian homemade cheeses, 14 (B1-B14) were selected based on their anti-Escherichia coli and anti-Staphylococcus aureus activities. These strains were also tested towards Listeria monocytogenes 161 and Salmonella Typhimurium LT2 and further investigated for their resistance to simulated gastrointestinal digestion, cell surface properties, ability to adhere to HT-29 cells, cholesterol lowering, antioxidant activity, and technological traits. Five isolates (B9, B13, B18, B19, and B38) were active against L. monocytogenes and Salmonella. From them, three isolates, identified as Lactobacillus brevis (B9, B13, and B38) by MALDI-TOF spectrometry and 16S rDNA sequencing, exhibited high tolerance to pancreatic juice, bile salts and acidic juices, high percentages of hydrophobicity (87, 92, and 81%, respectively), auto-aggregation (61, 68, and 72%, respectively), and adherence to HT-29 cells (79, 84, and 74%, respectively), which testify on their potential of colonization of the human intestine. On the other way, the strains B9 and B13 manifested the most relevant antioxidant activity and cholesterol-lowering ability, respectively. L. brevis strains showed low acidifying and good proteolytic activities with noticeable heat tolerance. The results gathered in this study highlighted the richness of Algerian artisanal cheeses on new lactobacilli strains with an excellent probiotic potential and demonstrated that L. brevis, largely used as nonstarter in cheese manufacture, could be exploited also as a probiotic for human use. |
| تدمد: | 1874-9356 0015-5632 |
| DOI: | 10.1007/s12223-021-00857-1 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::059bbcffb15fb72074aa0464e86c1181 https://doi.org/10.1007/s12223-021-00857-1 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....059bbcffb15fb72074aa0464e86c1181 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18749356 00155632 |
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| DOI: | 10.1007/s12223-021-00857-1 |