High-Fat Diet Enhances Neutrophil Adhesion in LDLR-Null Mice Via Hypercitrullination of Histone H3
| العنوان: | High-Fat Diet Enhances Neutrophil Adhesion in LDLR-Null Mice Via Hypercitrullination of Histone H3 |
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| المؤلفون: | Mizuko Osaka, Michiyo Deushi, Masayuki Yoshida, Jiro Aoyama, Akihito Ishigami, Tomoko Funakoshi |
| المصدر: | JACC: Basic to Translational Science |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, medicine.medical_specialty, HFD, high-fat diet, citrullination, Neutrophil adhesion, HUVECs, human umbilical vein endothelial cells, MPO, myeloperoxidase, PPAR, peroxisome proliferator-activated receptor, Femoral artery, 030204 cardiovascular system & hematology, NC, normal chow, 03 medical and health sciences, Histone H3, 0302 clinical medicine, NET, neutrophil extracellular trap, Internal medicine, medicine.artery, medicine, vascular inflammation, NE, neutrophil elastase, TG, triglyceride, Chemistry, Vascular inflammation, BW, body weight, TDFA, N-acetyl-l-threonyl-l-α-aspartyl-N5-(2-fluoro-1-iminoethyl)-l-ornithinamide trifluoroacetate salt, in vivo imaging, Citrullination, neutrophil, IVM, intravital microscopy, CXCL1, LysM, lysosome M, TC, total cholesterol, 030104 developmental biology, Endocrinology, DNaseI, deoxyribonuclease I, cxcl1, eGFP, enhanced green fluorescent protein, LDLR, low-density lipoprotein receptor, PAD4, peptidylarginine deiminase 4, LDL receptor, BM, bone marrow, Preclinical Research, wt, wild type, Cardiology and Cardiovascular Medicine, GM-CSF, granulocyte-macrophage colony-stimulating factor, Lipoprotein |
| الوصف: | Visual Abstract Highlights • Neutrophil adhesion to the vascular endothelium in the femoral artery of low-density lipoprotein receptor–null mice fed with HFD was significantly enhanced compared with wild-type mice fed with HFD or low-density lipoprotein receptor–null mice fed with NC. • HFD induced citrullinated histone H3 of neutrophils in LDL receptor–null mice through the up-regulation of CXCL1 in blood. • Hypercitrullination of histone H3 in neutrophils by CXCL1 enhanced neutrophil adhesion in vitro and in vivo. • Pemafibrate decreased neutrophil adhesion and citrullination of histone H3 through the reduction of CXCL1. Summary Neutrophil adhesion on the atheroprone femoral artery of high-fat diet–fed low-density lipoprotein receptor–null mice was enhanced more than in wild-type mice. The inhibition of histone H3 citrullination of neutrophils reversed the enhancement of neutrophil adhesion, suggesting that hypercitrullination contributes to enhanced neutrophil adhesion. Furthermore, pemafibrate reduced the citrullination of histone H3 in these mice. Therefore, the hypercitrullination of histone H3 in neutrophils contributes to atherosclerotic vascular inflammation. |
| تدمد: | 2452-302X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::04df94c59108ff2e67cbee057f800f21 https://pubmed.ncbi.nlm.nih.gov/34222722 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....04df94c59108ff2e67cbee057f800f21 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 2452302X |
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