The role of p53 and p21 on 8-chloro-adenosine-induced cellular response
| العنوان: | The role of p53 and p21 on 8-chloro-adenosine-induced cellular response |
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| المؤلفون: | Scott, Teakell, Lisa S, Chen, Christine M, Stellrecht, Varsha, Gandhi |
| المصدر: | Nucleosides, Nucleotides & Nucleic Acids. 41:1359-1374 |
| بيانات النشر: | Informa UK Limited, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Genetics, Molecular Medicine, General Medicine, Biochemistry |
| الوصف: | 8-Chloro-adenosine (8-Cl-Ado) is currently in phase I clinical trial. Activation of p53 and transactivation of p21 regulate cell fate after genotoxic insult. Using HCT-116-isogenic-cell-lines, we evaluated the role of p53/p21 after 8-Cl-Ado-mediated response. Following 30 µM 8-Cl-Ado treatment, RNA synthesis was inhibited, p53 protein was stabilized, and p21 expression was activated. None of the cell types were arrested in G1/S phase, however, cells lacking p53 were blocked in G2/M. These cells had the least increase in apoptotic cells, although clonogenic survival demonstrated equal inhibition in all 4 cell types. Collectively, irrespective of p53 and p21 status, 8-Cl-Ado-induced cytotoxicity was similar. |
| تدمد: | 1532-2335 1525-7770 |
| DOI: | 10.1080/15257770.2022.2038200 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::043fd873f19a1ec07c0bab0561cae795 https://doi.org/10.1080/15257770.2022.2038200 |
| رقم الانضمام: | edsair.doi.dedup.....043fd873f19a1ec07c0bab0561cae795 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15322335 15257770 |
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| DOI: | 10.1080/15257770.2022.2038200 |