Mycobacterium tuberculosis-infected alveolar epithelial cells modulate dendritic cell function through the HIF-1α-NOS2 axis
| العنوان: | Mycobacterium tuberculosis-infected alveolar epithelial cells modulate dendritic cell function through the HIF-1α-NOS2 axis |
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| المؤلفون: | Tamara S Rodrigues, Annie Rocio Piñeros Alvarez, Vânia Luiza Deperon Bonato, Niels Olsen Saraiva Câmara, José Carlos Farias Alves Filho, Bruno Marcel Silva de Melo, Ana Flávia Gembre, Maria Fernanda Forni |
| المصدر: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| بيانات النشر: | Oxford University Press (OUP), 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Tuberculosis, Immunology, Nitric Oxide Synthase Type II, Biology, Mycobacterium tuberculosis, Mice, Innate response, medicine, Animals, Immunology and Allergy, Glycolysis, Tuberculosis, Pulmonary, Mice, Knockout, Innate immune system, CÉLULAS EPITELIAIS, Dendritic Cells, Cell Biology, Dendritic cell, respiratory system, Hypoxia-Inducible Factor 1, alpha Subunit, Acquired immune system, medicine.disease, biology.organism_classification, Alveolar Epithelial Cells, Function (biology) |
| الوصف: | Tuberculosis kills more than 1 million people every year, and its control depends on the effective mechanisms of innate immunity, with or without induction of adaptive immune response. We investigated the interaction of type II alveolar epithelial cells (AEC-II) infected by Mycobacterium tuberculosis with dendritic cells (DCs). We hypothesized that the microenvironment generated by this interaction is critical for the early innate response against mycobacteria. We found that AEC-II infected by M. tuberculosis induced DC maturation, which was negatively regulated by HIF-1α-inducible NOS2 axis, and switched DC metabolism from an early and short peak of glycolysis to a low energetic status. However, the infection of DCs by M. tuberculosis up-regulated NOS2 expression and inhibited AEC-II-induced DC maturation. Our study demonstrated, for the first time, that HIF-1α-NOS2 axis plays a negative role in the maturation of DCs during M. tuberculosis infection. Such modulation might be useful for the exploitation of molecular targets to develop new therapeutic strategies against tuberculosis. |
| تدمد: | 1938-3673 0741-5400 |
| DOI: | 10.1002/jlb.3ma0520-113r |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0388aae5e7dfbd0bab19398ed472270a https://doi.org/10.1002/jlb.3ma0520-113r |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....0388aae5e7dfbd0bab19398ed472270a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19383673 07415400 |
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| DOI: | 10.1002/jlb.3ma0520-113r |