Cloning and Expression Analysis of MEP Pathway Enzyme-encoding Genes in Osmanthus fragrans
| العنوان: | Cloning and Expression Analysis of MEP Pathway Enzyme-encoding Genes in Osmanthus fragrans |
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| المؤلفون: | Lianggui Wang, Hongna Mu, Chunsun Gu, Huogeng Li, Chen Xu, Xiulian Yang, Yue Yuanzheng |
| المصدر: | Genes, Vol 7, Iss 10, p 78 (2016) Genes Genes; Volume 7; Issue 10; Pages: 78 |
| بيانات النشر: | MDPI AG, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0106 biological sciences, 0301 basic medicine, lcsh:QH426-470, 01 natural sciences, Article, 03 medical and health sciences, chemistry.chemical_compound, tissue-specific, Biosynthesis, Molecular evolution, Botany, flower development, Genetics, Gene, Genetics (clinical), Osmanthus fragrans, MEP pathway, diel oscillations, chemistry.chemical_classification, Osmanthus, Cloning, biology, biology.organism_classification, lcsh:Genetics, 030104 developmental biology, Enzyme, chemistry, Biochemistry, Function (biology), 010606 plant biology & botany |
| الوصف: | The 2-C-methyl-d-erythritol 4-phosphate (MEP) pathway is responsible for the biosynthesis of many crucial secondary metabolites, such as carotenoids, monoterpenes, plastoquinone, and tocopherols. In this study, we isolated and identified 10 MEP pathway genes in the important aromatic plant sweet osmanthus (Osmanthus fragrans). Multiple sequence alignments revealed that 10 MEP pathway genes shared high identities with other reported proteins. The genes showed distinctive expression profiles in various tissues, or at different flower stages and diel time points. The qRT-PCR results demonstrated that these genes were highly expressed in inflorescences, which suggested a tissue-specific transcript pattern. Our results also showed that OfDXS1, OfDXS2, and OfHDR1 had a clear diurnal oscillation pattern. The isolation and expression analysis provides a strong foundation for further research on the MEP pathway involved in gene function and molecular evolution, and improves our understanding of the molecular mechanism underlying this pathway in plants. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2073-4425 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::03466aff41a5a28512e67d214bb54dcd http://www.mdpi.com/2073-4425/7/10/78 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....03466aff41a5a28512e67d214bb54dcd |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20734425 |
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