The complex isotopologue space of glucose as a framework for the study of human intermediary metabolism
| العنوان: | The complex isotopologue space of glucose as a framework for the study of human intermediary metabolism |
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| المؤلفون: | Silke Marsch, Markus Fischer, Michael Vogeser, Tobias Gräwert, Dietrich Seidel, Adelbert Bacher, Ulrike Bacher, Harald Mückter, Christian Ettenhuber, Ralf Laupitz, Wolfgang Eisenreich |
| المصدر: | Isotopes in Environmental and Health Studies. 51:11-23 |
| بيانات النشر: | Informa UK Limited, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Blood Glucose, Male, Carbon Isotopes, Magnetic Resonance Spectroscopy, Chemistry, Citric Acid Cycle, Carbon-13, Pilot Projects, Carbon-13 NMR, Carbohydrate metabolism, Pentose phosphate pathway, Pentose Phosphate Pathway, Inorganic Chemistry, Citric acid cycle, chemistry.chemical_compound, Biochemistry, Humans, Environmental Chemistry, Glycolysis, Anaplerotic reactions, Isotopologue, General Environmental Science |
| الوصف: | The positional distributions of stable isotopes in metabolites provide specific fingerprints of the pathways and fluxes that have occurred in the organisms under study. In particular, modern nuclear magnetic resonance (NMR) spectroscopy enables the detailed assignment of isotope patterns in natural products, for example, in metabolites obtained from labelling experiments using (13)C-enriched precursors, such as glucose, acetate or CO2. In this study, the transient (13)C-isotopologue composition of blood glucose from an adult human volunteer after intravenous supply of [U-(13)C6]glucose was determined by high-resolution (13)C NMR spectroscopy. The non-linear progression curves displaying the relative amounts of eight (13)C-glucose isotopologues reflected the contributions of glucose metabolism by glycolytic cycling, the pentose phosphate pathway and anaplerotic reactions involving the citric acid cycle. The pilot study suggests that the experimental setting can be useful in analysing under non-invasive conditions the impact of physiological and pharmacological constraints on glucose turnover in humans. |
| تدمد: | 1477-2639 1025-6016 |
| DOI: | 10.1080/10256016.2014.1001845 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0340dec138cb2d0c90ecdf050eed4d88 https://doi.org/10.1080/10256016.2014.1001845 |
| رقم الانضمام: | edsair.doi.dedup.....0340dec138cb2d0c90ecdf050eed4d88 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14772639 10256016 |
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| DOI: | 10.1080/10256016.2014.1001845 |