Longitudinal Magnetic Resonance Imaging Analysis and Histological Characterization after Spinal Cord Injury in Two Mouse Strains with Different Functional Recovery: Gliosis as a Key Factor
| العنوان: | Longitudinal Magnetic Resonance Imaging Analysis and Histological Characterization after Spinal Cord Injury in Two Mouse Strains with Different Functional Recovery: Gliosis as a Key Factor |
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| المؤلفون: | Christophe Goze-Bac, Florence E. Perrin, Harun N. Noristani, Christophe Coillot, Guillaume P. Saint-Martin, Rahima Sidiboulenouar, Maida Cardoso, Matthias Catteau |
| المساهمون: | Institut des Neurosciences de Montpellier - Déficits sensoriels et moteurs (INM), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), Mécanismes moléculaires dans les démences neurodégénératives (MMDN), Université de Montpellier (UM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Santé et de la Recherche Médicale (INSERM)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL), Laboratoire Charles Coulomb (L2C), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier) |
| المصدر: | Journal of Neurotrauma Journal of Neurotrauma, Mary Ann Liebert, 2018, 35 (24), pp.2924-2940. ⟨10.1089/neu.2017.5613⟩ |
| بيانات النشر: | Mary Ann Liebert Inc, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Pathology, medicine.medical_specialty, Serotonergic, Lesion, Mice, recovery, 03 medical and health sciences, 0302 clinical medicine, In vivo, glial cell response to injury, medicine, Animals, Gliosis, Longitudinal Studies, Spinal cord injury, Spinal Cord Injuries, medicine.diagnostic_test, business.industry, Magnetic resonance imaging, Recovery of Function, Spinal cord, medicine.disease, Magnetic Resonance Imaging, spinal cord injury, Mice, Inbred C57BL, behavioral assessments, 030104 developmental biology, medicine.anatomical_structure, Neurology (clinical), medicine.symptom, business, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology, 030217 neurology & neurosurgery, Ex vivo, MRI |
| الوصف: | International audience; Spinal cord injuries (SCI) are disastrous neuropathologies causing permanent disabilities. The availability of different strains of mice is valuable for studying the pathophysiological mechanisms involved in SCI. However, strain differences have a profound effect on spontaneous functional recovery after SCI. CX3CR1+/eGFP and Aldh1l1-EGFP mice that express green fluorescent protein in microglia/monocytes and astrocytes, respectively, are particularly useful to study glial reactivity. Whereas CX3CR1+/eGFP mice have C57BL/6 background, Aldh1l1-EGFP are in Swiss Webster background. We first assessed spontaneous functional recovery in CX3CR1+/eGFP and Aldh1l1-EGFP mice over 6 weeks after lateral spinal cord hemisection. Second, we carried out a longitudinal follow-up of lesion evolution using in vivo T2-weighted magnetic resonance imaging (MRI). Finally, we performed in-depth analysis of the spinal cord tissue using ex vivo T2-weighted MRI as well as detailed histology. We demonstrate that CX3CR1+/eGFP mice have improved functional recovery and reduced anxiety after SCI compared with Aldh1l1-EGFP mice. We also found a strong correlation between in vivo MRI, ex vivo MRI, and histological analyses of the injured spinal cord in both strain of mice. All three modalities revealed no difference in lesion extension and volume between the two strains of mice. Importantly, histopathological analysis identified decreased gliosis and increased serotonergic axons in CX3CR1+/eGFP compared with Aldh1l1-EGFP mice following SCI. These results thus suggest that the strain-dependent improved functional recovery after SCI may be linked with reduced gliosis and increased serotonergic innervation. |
| تدمد: | 1557-9042 0897-7151 |
| DOI: | 10.1089/neu.2017.5613 |
| DOI: | 10.1089/neu.2017.5613⟩ |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::02bdc2946272423d84608d24ef701e72 https://doi.org/10.1089/neu.2017.5613 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....02bdc2946272423d84608d24ef701e72 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15579042 08977151 |
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| DOI: | 10.1089/neu.2017.5613 |