التفاصيل البيبلوغرافية
| العنوان: |
CD146 + cells are essential for kidney vasculature development |
| المؤلفون: |
Kimmo Halt, Susan E. Quaggin, Johanna Myllyharju, Seppo Vainio, Sunder Sims-Lucas, Sanna Junttila, Ulla Saarela, Heikki E. Pärssinen, Peppi Koivunen, Ilya Skovorodkin |
| المصدر: |
Kidney International. 90(2):311-324 |
| بيانات النشر: |
Elsevier BV, 2016. |
| سنة النشر: |
2016 |
| مصطلحات موضوعية: |
Vascular Endothelial Growth Factor A, 0301 basic medicine, podocyte, endothelium, Organogenesis, Fluorescent Antibody Technique, Kidney development, glomerulus, CD146 Antigen, Cell Separation, Biology, Kidney, Article, Mice, 03 medical and health sciences, Organ Culture Techniques, 0302 clinical medicine, Fate mapping, medicine, Animals, kidney development, Microscopy, Video, Regeneration (biology), Endothelial Cells, Kinase insert domain receptor, Flow Cytometry, Vascular Endothelial Growth Factor Receptor-2, Embryonic stem cell, Cell biology, Mice, Inbred C57BL, Platelet Endothelial Cell Adhesion Molecule-1, Vascular endothelial growth factor A, 030104 developmental biology, medicine.anatomical_structure, Nephrology, Immunology, cardiovascular system, CD146, erythropoietin, 030217 neurology & neurosurgery, Signal Transduction |
| الوصف: |
The kidney vasculature is critical for renal function, but its developmental assembly mechanisms remain poorly understood and models for studying its assembly dynamics are limited. Here, we tested whether the embryonic kidney contains endothelial cells (ECs) that are heterogeneous with respect to VEGFR2/Flk1/KDR, CD31/PECAM, and CD146/MCAM markers. Tie1Cre;R26R YFP -based fate mapping with a time-lapse in embryonic kidney organ culture successfully depicted the dynamics of kidney vasculature development and the correlation of the process with the CD31 + EC network. Depletion of Tie1 + or CD31 + ECs from embryonic kidneys, with either Tie1Cre- induced diphtheria toxin susceptibility or cell surface marker–based sorting in a novel dissociation and reaggregation technology, illustrated substantial EC network regeneration. Depletion of the CD146 + cells abolished this EC regeneration. Fate mapping of green fluorescent protein (GFP)-marked CD146 + /CD31 - cells indicated that they became CD31 + cells, which took part in EC structures with CD31 + wild-type ECs. EC network development depends on VEGF signaling, and VEGF and erythropoietin are expressed in the embryonic kidney even in the absence of any external hypoxic stimulus. Thus, the ex vivo embryonic kidney culture models adopted here provided novel ways for targeting renal EC development and demonstrated that CD146 + cells are critical for kidney vasculature development. |
| تدمد: |
0085-2538 |
| DOI: |
10.1016/j.kint.2016.02.021 |
| URL الوصول: |
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0188072332b96382746df67b1c6f2112 |
| Rights: |
OPEN |
| رقم الانضمام: |
edsair.doi.dedup.....0188072332b96382746df67b1c6f2112 |
| قاعدة البيانات: |
OpenAIRE |