Comparative pulmonary toxicity of a DWCNT and MWCNT-7 in rats
| العنوان: | Comparative pulmonary toxicity of a DWCNT and MWCNT-7 in rats |
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| المؤلفون: | Takamasa Numano, Aya Naiki, Akihiko Hirose, William T. Alexander, Jun Kannno, David B. Alexander, Masaaki Iigo, Satoru Takahashi, Hiroshi Takase, Ashraf Mohamed Nazem, Hiroyuki Tsuda, Mohamed Abdelgied, Osama Saeid Elokle, Ahmed M. El-Gazzar |
| المصدر: | Archives of Toxicology |
| بيانات النشر: | Springer Berlin Heidelberg, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Male, Pathology, medicine.medical_specialty, Double-walled carbon nanotubes, Pulmonary toxicity, Health, Toxicology and Mutagenesis, 010501 environmental sciences, Toxicology, 01 natural sciences, 03 medical and health sciences, chemistry.chemical_compound, DNA Adducts, Lactate dehydrogenase, Macrophages, Alveolar, Toxicity Tests, medicine, Animals, Nanotoxicology, 0105 earth and related environmental sciences, Inhalation Exposure, Lung, biology, L-Lactate Dehydrogenase, Chemistry, Nanotubes, Carbon, Granulation tissue, General Medicine, Pleural cavity, Intra-tracheal intra-pulmonary spraying, Alkaline Phosphatase, Fibrosis, Rats, Inbred F344, Proliferating cell nuclear antigen, Pulmonary Alveoli, 030104 developmental biology, medicine.anatomical_structure, Toxicity, biology.protein, Alkaline phosphatase, Inhalation toxicity, Rat, TIPS, DWCNT, Chemokines, Bronchoalveolar Lavage Fluid |
| الوصف: | Very little is known about the in vivo toxicity of inhaled double-walled carbon nanotubes (DWCNTs). In the present study, we compared the pulmonary toxicity of DWCNT to MWCNT-7, a well-known multi-walled carbon nanotube. Rats were divided into six groups: untreated, vehicle, low-dose DWCNT, high-dose DWCNT, low-dose MWCNT-7, and high-dose MWCNT-7. The test materials were administered by intra-tracheal intra-pulmonary spraying (TIPS) every other day for 15 days: the low-dose and high-dose groups were administered final total doses of 0.25 and 0.50 mg/rat of the test material. The animals were sacrificed 1 and 6 weeks after the final TIPS administration. Six weeks after the final TIPS administration, rats administered MWCNT-7 had high levels of macrophage infiltration into the lung with dense alveolar wall fibrous thickening throughout the lung; significant elevation of lactate dehydrogenase activity, alkaline phosphatase activity, and total protein concentration in the bronchioalveolar lavage fluid; an increase in the pulmonary cell PCNA index; slightly elevated levels of 8-OHdG DNA adducts in lung tissue DNA; a small but significant increase in protein concentration in the pleural cavity lavage fluid and an increase in the visceral mesothelial cell PCNA index. None of these parameters was increased in rats administered DWCNT. The primary lesion in rats administered DWCNT was scattered formation of granulation tissue containing internalized DWCNT fibers. Our data indicate that DWCNT has lower pulmonary and pleural toxicity than MWCNT-7. Electronic supplementary material The online version of this article (10.1007/s00204-018-2336-3) contains supplementary material, which is available to authorized users. |
| اللغة: | English |
| تدمد: | 1432-0738 0340-5761 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::016d2370d2c8fda801bb3b62d5719f8b http://europepmc.org/articles/PMC6343020 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....016d2370d2c8fda801bb3b62d5719f8b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14320738 03405761 |
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