Investigation of cholestasis‐related changes in characteristics of spermatogonial stem cells in testis tissue of male Wistar rats
| العنوان: | Investigation of cholestasis‐related changes in characteristics of spermatogonial stem cells in testis tissue of male Wistar rats |
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| المؤلفون: | Amir Hossein Hasani Fard, Homa Mohseni Kouchesfehani, Hanieh Jalali |
| المصدر: | Andrologia. 52 |
| بيانات النشر: | Hindawi Limited, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Male, endocrine system, Offspring, Urology, 030232 urology & nephrology, Biology, Andrology, 03 medical and health sciences, 0302 clinical medicine, Endocrinology, Cholestasis, In vivo, Testis, medicine, Animals, Spermatogonial stem cells, Rats, Wistar, Spermatogenesis, Cells, Cultured, 030219 obstetrics & reproductive medicine, Adult Germline Stem Cells, Metabolic disorder, General Medicine, medicine.disease, Spermatogonia, In vitro, Rats, Immunostaining |
| الوصف: | Paternal metabolic status is an important factor in the health status of offspring. Cholestasis, as a metabolic disorder, significantly disrupts spermatogenesis. Spermatogonial stem cells (SSCs) are considered the dividing germ cells, which maintain spermatogenesis throughout the lifespan. Here, we investigated the in vivo and in vitro effect(s) of cholestasis on SSCs. Cholestasis was induced in rats by bile duct ligation. Four weeks after the cholestasis induction, testicular tissues were analysed using histopathological examinations. The expression of SSC markers, including Plzf and Thy-1, was determined using the immunofluorescent technique. Also, SSCs were isolated from animals, and their proliferation was examined in vitro. The histological examinations revealed that cholestasis caused irregularities in the structure of seminal tubes. Immunostaining showed that the total number of Thy-1- and Plzf-expressing cells was declined in the cholestasis group compared with the control group. In vitro culture of SSCs indicated that the number of SSC colonies and those expressing Plzf were significantly reduced in the culture medium of the cholestasis group. Our results indicated that cholestasis affects the functionality of SSCs and reduces the number and proliferation of them. This finding may be of interest to the effect of metabolic diseases such as cholestasis on spermatogenesis. |
| تدمد: | 1439-0272 0303-4569 |
| DOI: | 10.1111/and.13660 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::00f1012070b79c5412842a3fcee5e75c https://doi.org/10.1111/and.13660 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....00f1012070b79c5412842a3fcee5e75c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14390272 03034569 |
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| DOI: | 10.1111/and.13660 |