Shear‐dependent platelet aggregation size
| العنوان: | Shear‐dependent platelet aggregation size |
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| المؤلفون: | Katrina K Ki, Geoff Tansley, Tomotaka Murashige, Nobuo Watanabe, Masataka Inoue, John F. Fraser, Chris H H Chan, Michael J. Simmonds |
| المصدر: | Artificial Organs |
| بيانات النشر: | Wiley, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Blood Platelets, exposure time, Platelet Aggregation, 0206 medical engineering, Biomedical Engineering, ADAMTS13 Protein, Medicine (miscellaneous), Bioengineering, 02 engineering and technology, Postoperative Hemorrhage, von Willebrand factor, 030204 cardiovascular system & hematology, Risk Assessment, Prosthesis Implantation, Biomaterials, 03 medical and health sciences, shear rate, 0302 clinical medicine, Von Willebrand factor, Main Text Articles, Disintegrin, Humans, Platelet, Gel electrophoresis, Thrombospondin, Main Text Article, biology, Chemistry, Impaired platelet aggregation, General Medicine, platelet aggregate, ADAMTS13, 020601 biomedical engineering, Healthy Volunteers, Molecular Weight, Shear rate, biology.protein, Biophysics, Heart-Assist Devices, Stress, Mechanical, Protein Multimerization |
| الوصف: | Nonsurgical bleeding is the most frequent complication of left ventricular assist device (LVAD) support. Supraphysiologic shear rates generated in LVAD causes impaired platelet aggregation, which increases the risk of bleeding. The effect of shear rate on the formation size of platelet aggregates has never been reported experimentally, although platelet aggregation size can be considered to be directly relevant to bleeding complications. Therefore, this study investigated the impact of shear rate and exposure time on the formation size of platelet aggregates, which is vital in predicting bleeding in patients with an LVAD. Human platelet‐poor plasma (containing von Willebrand factor, vWF) and fluorochrome‐labeled platelets were subjected to a range of shear rates (0‐10 000 s−1) for 0, 5, 10, and 15 minutes using a custom‐built blood‐shearing device. Formed sizes of platelet aggregates under a range of shear‐controlled environment were visualized and measured using microscopy. The loss of high molecular weight (HMW) vWF multimers was quantified using gel electrophoresis and immunoblotting. An inhibition study was also performed to investigate the reduction in platelet aggregation size and HMW vWF multimers caused by either mechanical shear or enzymatic (a disintegrin and metalloproteinase with a thrombospondin type 1 motif, member 13—ADAMTS13, the von Willebrand factor protease) mechanism under low and high shear conditions (360 and 10 000 s−1). We found that the average size of platelet aggregates formed under physiological shear rates of 360‐3000 s−1 (200‐300 μm2) was significantly larger compared to those sheared at >6000 s−1 (50‐100 μm2). Furthermore, HMW vWF multimers were reduced with increased shear rates. The inhibition study revealed that the reduction in platelet aggregation size and HWM vWF multimers were mainly associated with ADAMTS13. In conclusion, the threshold of shear rate must not exceed >6000 s−1 in order to maintain the optimal size of platelet aggregates to “plug off” the injury site and stop bleeding. |
| تدمد: | 1525-1594 0160-564X |
| DOI: | 10.1111/aor.13783 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::00610ae6ff4febbdaf93827f953a165b https://doi.org/10.1111/aor.13783 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....00610ae6ff4febbdaf93827f953a165b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15251594 0160564X |
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| DOI: | 10.1111/aor.13783 |